Leakage-free bonding of porous membranes into layered microfluidic array systems

Bor Han Chueh, Dongeun Huh, Christina R. Kyrtsos, Timothée Houssin, Nobuyuki Futai, Shuichi Takayama

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The integration of semiporous membranes into poly-(dimethylsiloxane) (PDMS) microfluidic devices is useful for mass transport control. Several methods such as plasma oxidation and manual application of PDMS prepolymer exist to sandwich such membranes into simple channel structures, but these methods are difficult to implement with reliable sealing and no leakage or clogging for devices with intricate channel features. This paper describes a simple but robust strategy to bond semiporous polyester and polycarbonate membranes between layers of PDMS microchannel structures effectively without channel clogging. A thin layer of PDMS prepolymer, spin-coated on a glass slide, is transferred to PDMS substrates with channel features as well as to the edges of the semiporous membrane by stamping. This thin PDMS prepolymer serves as "mortar" to strongly bond the two PDMS layers and seal off the crevices generated from the thickness of the membranes. This bonding method enabled the fabrication of an 8 x 12 criss-crossing microfluidic channel array with 96 combinations of fluid interactions. The capability of this device for bioanalysis was demonstrated by measuring responses of cells to different color fluorescent reagents.

Original languageEnglish
Pages (from-to)3504-3508
Number of pages5
JournalAnalytical Chemistry
Volume79
Issue number9
DOIs
Publication statusPublished - 2007 May 1
Externally publishedYes

Fingerprint

Microfluidics
Membranes
polycarbonate
Polyesters
Stamping
baysilon
Microchannels
Mortar
Seals
Mass transfer
Color
Plasmas
Fabrication
Glass
Oxidation
Fluids
Substrates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Chueh, B. H., Huh, D., Kyrtsos, C. R., Houssin, T., Futai, N., & Takayama, S. (2007). Leakage-free bonding of porous membranes into layered microfluidic array systems. Analytical Chemistry, 79(9), 3504-3508. https://doi.org/10.1021/ac062118p

Leakage-free bonding of porous membranes into layered microfluidic array systems. / Chueh, Bor Han; Huh, Dongeun; Kyrtsos, Christina R.; Houssin, Timothée; Futai, Nobuyuki; Takayama, Shuichi.

In: Analytical Chemistry, Vol. 79, No. 9, 01.05.2007, p. 3504-3508.

Research output: Contribution to journalArticle

Chueh, BH, Huh, D, Kyrtsos, CR, Houssin, T, Futai, N & Takayama, S 2007, 'Leakage-free bonding of porous membranes into layered microfluidic array systems', Analytical Chemistry, vol. 79, no. 9, pp. 3504-3508. https://doi.org/10.1021/ac062118p
Chueh, Bor Han ; Huh, Dongeun ; Kyrtsos, Christina R. ; Houssin, Timothée ; Futai, Nobuyuki ; Takayama, Shuichi. / Leakage-free bonding of porous membranes into layered microfluidic array systems. In: Analytical Chemistry. 2007 ; Vol. 79, No. 9. pp. 3504-3508.
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