Local ablation by micro-electric knife

Yoko Yamanishi, Hiroki Kuriki, Shinya Sakuma, Kazuhisa Onda, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have firstly succeeded in enucleation of oocyte by using micro-electric knife. The discharged output power and conductive area were controlled to adapt the cellular-order ablation under water and under atmospheric pressure environment. The local area ablation was obtained by the glass shell insulation around the copper wire with silver paste, and which prevent the bubble generation when it was discharged under the water. This fabricated sharp electric knife has a designed small space at the end of the knife between the wire and the glass tip, and which contribute to stabilize the electric discharge. The width of the ablation region was successfully reduced to more than a half compare to the conventional wire electrode, by using the robust glass shell insulation and a single micro-scale bubble injected from inside of the electrode. Moreover, the nucleus dyed by Hoechst was also removed successfully with limited damage region which is the difficult task for manual operation with glass capillary. This low cost micro-electric knife can fabricate any objective material under various environments and it has a possibility to contribute to a new fabrication method in micro-nano bioengineering field.

Original languageEnglish
Title of host publication2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages129-132
Number of pages4
DOIs
Publication statusPublished - 2012 Jun 1
Event7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
Duration: 2012 Mar 52012 Mar 8

Publication series

Name2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Conference

Conference7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
CountryJapan
CityKyoto
Period12/3/512/3/8

Keywords

  • cell surgery
  • electric knife
  • formatting
  • micro-ablation

ASJC Scopus subject areas

  • Engineering (miscellaneous)

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  • Cite this

    Yamanishi, Y., Kuriki, H., Sakuma, S., Onda, K., & Arai, F. (2012). Local ablation by micro-electric knife. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 129-132). [6196739] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196739