Long range surface plasmon-coupled fluorescence emission for biosensor applications

Koji Toma, Wolfgang Knoll, Jakub Dostalek

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A biosensor scheme that employs long range surface plasmons (LRSPs) for the efficient excitation and collection of fluorescence light from fluorophore-labeled biomolecules captured in a three-dimensional hydrogel matrix is discussed. This new approach to plasmon-enhanced fluorescence (PEF) is experimentally and theoretically investigated by using the Kretschmann configuration of attenuated total reflection (ATR) method. A layer structure supporting LRSPs that consists of a low refractive index fluoropolymer layer, a thin gold film and a large binding capacity N-isopropylacrylamide (NIPAAm) hydrogel matrix is employed. By using this layer architecture, the extended field of LRSPs can probe the binding of biomolecules at up to micrometer distances from the gold surface. With respect to regular surface plasmon-enhanced fluorescence spectroscopy (SPFS) and surface plasmon-coupled emission (SPCE), a narrower angular distribution of the fluorescence light intensity, larger peak intensity and the excitation and emission at lower angles were observed.

Original languageEnglish
Title of host publication2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event2011 International Workshop on Biophotonics, BIOPHOTONICS 2011 - Parma, Italy
Duration: 2011 Jun 82011 Jun 10

Publication series

Name2011 International Workshop on Biophotonics, BIOPHOTONICS 2011

Conference

Conference2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
Country/TerritoryItaly
CityParma
Period11/6/811/6/10

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Biomedical Engineering

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