MALC, a novel microinjection method for loading of macromolecules into cultured neurons

Ryoji Yano, Daisuke Okada, Chan Choo Yap, Kunihiko Mabuchi, Hitoshi Muguruma, Takashi K. Saito

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microinjection is one of the most useful and important methods in cellular neurobiology. However, direct insertion and retraction of a capillary produce physical stresses inside cells that make it difficult to apply on small and fragile cells, especially the central nervous system (CNS) neurons. In this study, we developed a novel method called MALC (microinjection method assisted with laser activated chromophore) to avoid this disadvantage of the conventional method. In MALC, a capillary is just attached on cell surface, and combination of a newly developed photosensitizer with a laser pointing system makes the plasma membrane permeable to molecules. This novel method can expand the application of the microinjection method and realize molecular manipulations inside CNS neurons.

Original languageEnglish
Pages (from-to)1263-1266
Number of pages4
JournalNeuroReport
Volume13
Issue number10
DOIs
Publication statusPublished - 2002 Jul 19

Keywords

  • BAT
  • Ca indicator
  • Chromophore
  • Laser
  • MALC
  • Microinjection

ASJC Scopus subject areas

  • Neuroscience(all)

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