Measurement of single low-density lipoprotein particles by atomic force microscopy

Toshihiro Sakurai; Seiji Takeda; Jun Ya Takahashi; Yuji Takahashi; Norio Wada; Suchin Trirongjitmoah; Takeshi Namita; Shigeki Jin; Akiko Ikuta; Hiroaki Furumaki; Shu Ping Hui; Hirotoshi Fuda; Masato Fujikawa; Koichi Shimizu; Hitoshi Chiba

doi:10.1177/0004563213481586

Measurement of single low-density lipoprotein particles by atomic force microscopy

Toshihiro Sakurai, Seiji Takeda, Jun Ya Takahashi, Yuji Takahashi, Norio Wada, Suchin Trirongjitmoah, Takeshi Namita, Shigeki Jin, Akiko Ikuta, Hiroaki Furumaki, Shu Ping Hui, Hirotoshi Fuda, Masato Fujikawa, Koichi Shimizu, Hitoshi Chiba

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P < 0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P < 0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P < 0.05 for large LDL and P < 0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

Original language	English
Pages (from-to)	564-570
Number of pages	7
Journal	Annals of Clinical Biochemistry
Volume	50
Issue number	6
DOIs	https://doi.org/10.1177/0004563213481586
Publication status	Published - 2013 Nov
Externally published	Yes

Keywords

AFM
Electron microscopy
Large LDL
Small dense LDL

ASJC Scopus subject areas

Clinical Biochemistry

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10.1177/0004563213481586

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Sakurai, T., Takeda, S., Takahashi, J. Y., Takahashi, Y., Wada, N., Trirongjitmoah, S., Namita, T., Jin, S., Ikuta, A., Furumaki, H., Hui, S. P., Fuda, H., Fujikawa, M., Shimizu, K., & Chiba, H. (2013). Measurement of single low-density lipoprotein particles by atomic force microscopy. Annals of Clinical Biochemistry, 50(6), 564-570. https://doi.org/10.1177/0004563213481586

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title = "Measurement of single low-density lipoprotein particles by atomic force microscopy",

abstract = "Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P < 0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P < 0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P < 0.05 for large LDL and P < 0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.",

keywords = "AFM, Electron microscopy, Large LDL, Small dense LDL",

author = "Toshihiro Sakurai and Seiji Takeda and Takahashi, {Jun Ya} and Yuji Takahashi and Norio Wada and Suchin Trirongjitmoah and Takeshi Namita and Shigeki Jin and Akiko Ikuta and Hiroaki Furumaki and Hui, {Shu Ping} and Hirotoshi Fuda and Masato Fujikawa and Koichi Shimizu and Hitoshi Chiba",

note = "Funding Information: This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and also by the Regional Innovation Strategy Support Program of the Ministry of Education, Culture, Sports, Science and Technology, Japan. ",

year = "2013",

month = nov,

doi = "10.1177/0004563213481586",

language = "English",

volume = "50",

pages = "564--570",

journal = "Annals of Clinical Biochemistry",

issn = "0004-5632",

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TY - JOUR

T1 - Measurement of single low-density lipoprotein particles by atomic force microscopy

AU - Sakurai, Toshihiro

AU - Takeda, Seiji

AU - Takahashi, Jun Ya

AU - Takahashi, Yuji

AU - Wada, Norio

AU - Trirongjitmoah, Suchin

AU - Namita, Takeshi

AU - Jin, Shigeki

AU - Ikuta, Akiko

AU - Furumaki, Hiroaki

AU - Hui, Shu Ping

AU - Fuda, Hirotoshi

AU - Fujikawa, Masato

AU - Shimizu, Koichi

AU - Chiba, Hitoshi

N1 - Funding Information: This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and also by the Regional Innovation Strategy Support Program of the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2013/11

Y1 - 2013/11

N2 - Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P < 0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P < 0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P < 0.05 for large LDL and P < 0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

AB - Background: The size of lipoprotein particles is relevant to the risk of coronary artery disease (CAD). Methods: We investigated the feasibility of atomic force microscopy (AFM) for evaluating the size of large low-density lipoprotein (LDL) and small dense LDL (sd-LDL) separated by ultracentrifugation. The measurements by AFM in tapping mode were compared to those by electron microscopy (EM). Results: There was a significant difference in particle sizes determined by AFM between large LDL (20.6±1.9 nm, mean SD) and sd-LDL (16.2±1.4 nm) obtained from six healthy volunteers (P < 0.05). The particle sizes determined by EM for the same samples were 23.2±1.4 nm for large LDL and 20.4±1.4 nm for sd-LDL. The difference between large LDL and sd-LDL detected by EM was also statistically significant (P < 0.05). In addition, the particle sizes of each lipoprotein fraction were significantly different between AFM and EM: P < 0.05 for large LDL and P < 0.05 for sd-LDL. Conclusions: AFM can differentiate between sd-LDL and large LDL particles by their size, and might be useful for evaluating risk for CAD.

KW - AFM

KW - Electron microscopy

KW - Large LDL

KW - Small dense LDL

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EP - 570

JO - Annals of Clinical Biochemistry

JF - Annals of Clinical Biochemistry

IS - 6

ER -

Measurement of single low-density lipoprotein particles by atomic force microscopy

Abstract

Keywords

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