Mechanism of amperometric biosensor with electronic-type-controlled carbon nanotube

Hiroki Hidaka, Kohei Nowaki, Hitoshi Muguruma

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An amperometric enzyme biosensor with electronic-type-controlled (metallic and semiconducting) single-walled carbon nanotubes (CNTs) is presented. In this research, we investigate how the electronic types of CNTs influence the amperometric response of enzyme biosensors and what their working mechanisms are. The biosensor of interest is for glucose detection using enzyme glucose oxidase (GOD). In the presence of oxygen, the response of a metallic CNT-GOD electrode was 2.5 times more sensitive than that of a semiconducting CNT-GOD electrode. In contrast, in the absence of oxygen, the response of the semiconducting CNT-GOD electrode was retained, whereas that of the metallic CNT-GOD electrode was significantly reduced. This indicates that direct electron transfer occurred with the semiconducting CNT-GOD electrode, whereas the metallic CNTGOD electrode was dominated by a hydrogen peroxide pathway caused by an enzymatic reaction. Electrochemical impedance spectroscopy was used to show that the semiconducting CNT network has less resistance for electron transfer than the metallic CNT network. The optimized glucose biosensor revealed a sensitivity of 5.6 ?AmM%1cm%2 at +0.6 V vs Ag/AgCl, a linear dynamic range of 0.025-1.4 mM, and a response time of 8 s.

Original languageEnglish
Article number03DF01
JournalJapanese Journal of Applied Physics
Volume55
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

Fingerprint

bioinstrumentation
Biosensors
Glucose oxidase
glucose
Carbon nanotubes
oxidase
carbon nanotubes
electronics
Electrodes
electrodes
Enzymes
enzymes
Glucose
electron transfer
Oxygen
Electrons
Single-walled carbon nanotubes (SWCN)
Electrochemical impedance spectroscopy
Hydrogen peroxide
oxygen

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Mechanism of amperometric biosensor with electronic-type-controlled carbon nanotube. / Hidaka, Hiroki; Nowaki, Kohei; Muguruma, Hitoshi.

In: Japanese Journal of Applied Physics, Vol. 55, No. 3, 03DF01, 01.03.2016.

Research output: Contribution to journalArticle

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