Method for the determination of vitamin K homologues in human plasma using high-performance liquid chromatography-tandem mass spectrometry

Yoshitomo Suhara, Maya Kamao, Naoko Tsugawa, Toshio Okano

Research output: Contribution to journalArticle

82 Citations (Scopus)


We report here the development of a precise and sensitive method for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7) in human plasma using HPLC-tandem mass-mass spectrometry with atmospheric pressure chemical ionization (LC-APCI-MS/MS). The method involves the use of stable isotope 18O-labeled internal standard compounds, which were synthesized in our laboratory, and the selection of a precursor and product ion with a MS/MS multiple reaction monitoring method. The average intraassay and interassay variation values for PK, MK-4, and MK-7 were <10%. Average spiked recoveries from authentic compounds added to normal human plasma samples for PK, MK-4, and MK-7 were 98-102%. Mean plasma concentrations of PK, MK-4, and MK-7 from healthy subjects (n = 20) were 1.22 ± 0.57, 0.39 ± 0.46, and 6.37 ± 7.45 ng/mL, respectively. We conclude that this novel LC-APCI-MS/MS method should be useful for the evaluation of vitamin K status in postmenopausal women and elderly subjects and provides useful information for the treatment and prevention of osteoporosis with vitamin K.

Original languageEnglish
Pages (from-to)757-763
Number of pages7
JournalAnalytical Chemistry
Issue number3
Publication statusPublished - 2005 Feb 1


ASJC Scopus subject areas

  • Analytical Chemistry

Cite this