Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments

Hisako Hirayama, Hiroyuki Fuse, Mariko Abe, Masayuki Miyazaki, Takamichi Nakamura, Takuro Nunoura, Yasuo Furushima, Hiroyuki Yamamoto, Ken Takai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4 T and T2-1 were Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum and type I intracytoplasmic membranes. They were obligate methanotrophs that grew only on methane or methanol. Each strain possessed the particulate methane monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. The strains grew best at 37 °C, and did not grow at 45 °C. NaCl was required for growth within a concentration range of 1-8 % (w/v). The major phospholipid fatty acids were C 16: 0, C 16: 1ω7c, and C 16: 1ω5t. The major isoprenoid quinone was MQ-8. The DNA G+C content was 50.9-51.7 mol%. The 16S rRNA gene sequences of the strains showed 99.4 % similarity to each other, and DNA-DNA hybridization analysis indicated that the strains were representatives of the same species. The 16S rRNA gene sequences were highly similar to some marine environmental sequences (94.0-97.7 % similarity), but did not show similarities more than 94 % with sequences of members of other related genera, such as Methylomicrobium, Methylobacter, Methylomonas and Methylosarcina. Phylogenies based on 16S rRNA gene sequences and deduced partial PmoA sequences, and the physiological and chemotaxonomic characteristics revealed that strains IT-4 T and T2-1 represent a novel species of a new genus in the family Methylococcaceae, for which the name Methylomarinum vadi gen. nov., sp. nov. is proposed. The type strain is IT-4 T (= JCM 13665 T = DSM 18976 T).

Original languageEnglish
Pages (from-to)1073-1082
Number of pages10
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART3
DOIs
Publication statusPublished - 2013

Fingerprint

methanotrophs
marine environment
methane
DNA
gene
new species
isoprenoid
phospholipid
new genus
methanol
ribosomal RNA
phylogeny
fatty acid
nucleotide sequences
membrane
Methylococcaceae
Methylomicrobium
Methylosarcina
Methylomonas
Methylobacter

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments. / Hirayama, Hisako; Fuse, Hiroyuki; Abe, Mariko; Miyazaki, Masayuki; Nakamura, Takamichi; Nunoura, Takuro; Furushima, Yasuo; Yamamoto, Hiroyuki; Takai, Ken.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART3, 2013, p. 1073-1082.

Research output: Contribution to journalArticle

Hirayama, H, Fuse, H, Abe, M, Miyazaki, M, Nakamura, T, Nunoura, T, Furushima, Y, Yamamoto, H & Takai, K 2013, 'Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments', International Journal of Systematic and Evolutionary Microbiology, vol. 63, no. PART3, pp. 1073-1082. https://doi.org/10.1099/ijs.0.040568-0
Hirayama, Hisako ; Fuse, Hiroyuki ; Abe, Mariko ; Miyazaki, Masayuki ; Nakamura, Takamichi ; Nunoura, Takuro ; Furushima, Yasuo ; Yamamoto, Hiroyuki ; Takai, Ken. / Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. PART3. pp. 1073-1082.
@article{604d677c5c164f469cce508658ee440e,
title = "Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments",
abstract = "Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4 T and T2-1 were Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum and type I intracytoplasmic membranes. They were obligate methanotrophs that grew only on methane or methanol. Each strain possessed the particulate methane monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. The strains grew best at 37 °C, and did not grow at 45 °C. NaCl was required for growth within a concentration range of 1-8 {\%} (w/v). The major phospholipid fatty acids were C 16: 0, C 16: 1ω7c, and C 16: 1ω5t. The major isoprenoid quinone was MQ-8. The DNA G+C content was 50.9-51.7 mol{\%}. The 16S rRNA gene sequences of the strains showed 99.4 {\%} similarity to each other, and DNA-DNA hybridization analysis indicated that the strains were representatives of the same species. The 16S rRNA gene sequences were highly similar to some marine environmental sequences (94.0-97.7 {\%} similarity), but did not show similarities more than 94 {\%} with sequences of members of other related genera, such as Methylomicrobium, Methylobacter, Methylomonas and Methylosarcina. Phylogenies based on 16S rRNA gene sequences and deduced partial PmoA sequences, and the physiological and chemotaxonomic characteristics revealed that strains IT-4 T and T2-1 represent a novel species of a new genus in the family Methylococcaceae, for which the name Methylomarinum vadi gen. nov., sp. nov. is proposed. The type strain is IT-4 T (= JCM 13665 T = DSM 18976 T).",
author = "Hisako Hirayama and Hiroyuki Fuse and Mariko Abe and Masayuki Miyazaki and Takamichi Nakamura and Takuro Nunoura and Yasuo Furushima and Hiroyuki Yamamoto and Ken Takai",
year = "2013",
doi = "10.1099/ijs.0.040568-0",
language = "English",
volume = "63",
pages = "1073--1082",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "PART3",

}

TY - JOUR

T1 - Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments

AU - Hirayama, Hisako

AU - Fuse, Hiroyuki

AU - Abe, Mariko

AU - Miyazaki, Masayuki

AU - Nakamura, Takamichi

AU - Nunoura, Takuro

AU - Furushima, Yasuo

AU - Yamamoto, Hiroyuki

AU - Takai, Ken

PY - 2013

Y1 - 2013

N2 - Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4 T and T2-1 were Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum and type I intracytoplasmic membranes. They were obligate methanotrophs that grew only on methane or methanol. Each strain possessed the particulate methane monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. The strains grew best at 37 °C, and did not grow at 45 °C. NaCl was required for growth within a concentration range of 1-8 % (w/v). The major phospholipid fatty acids were C 16: 0, C 16: 1ω7c, and C 16: 1ω5t. The major isoprenoid quinone was MQ-8. The DNA G+C content was 50.9-51.7 mol%. The 16S rRNA gene sequences of the strains showed 99.4 % similarity to each other, and DNA-DNA hybridization analysis indicated that the strains were representatives of the same species. The 16S rRNA gene sequences were highly similar to some marine environmental sequences (94.0-97.7 % similarity), but did not show similarities more than 94 % with sequences of members of other related genera, such as Methylomicrobium, Methylobacter, Methylomonas and Methylosarcina. Phylogenies based on 16S rRNA gene sequences and deduced partial PmoA sequences, and the physiological and chemotaxonomic characteristics revealed that strains IT-4 T and T2-1 represent a novel species of a new genus in the family Methylococcaceae, for which the name Methylomarinum vadi gen. nov., sp. nov. is proposed. The type strain is IT-4 T (= JCM 13665 T = DSM 18976 T).

AB - Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4 T and T2-1 were Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum and type I intracytoplasmic membranes. They were obligate methanotrophs that grew only on methane or methanol. Each strain possessed the particulate methane monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. The strains grew best at 37 °C, and did not grow at 45 °C. NaCl was required for growth within a concentration range of 1-8 % (w/v). The major phospholipid fatty acids were C 16: 0, C 16: 1ω7c, and C 16: 1ω5t. The major isoprenoid quinone was MQ-8. The DNA G+C content was 50.9-51.7 mol%. The 16S rRNA gene sequences of the strains showed 99.4 % similarity to each other, and DNA-DNA hybridization analysis indicated that the strains were representatives of the same species. The 16S rRNA gene sequences were highly similar to some marine environmental sequences (94.0-97.7 % similarity), but did not show similarities more than 94 % with sequences of members of other related genera, such as Methylomicrobium, Methylobacter, Methylomonas and Methylosarcina. Phylogenies based on 16S rRNA gene sequences and deduced partial PmoA sequences, and the physiological and chemotaxonomic characteristics revealed that strains IT-4 T and T2-1 represent a novel species of a new genus in the family Methylococcaceae, for which the name Methylomarinum vadi gen. nov., sp. nov. is proposed. The type strain is IT-4 T (= JCM 13665 T = DSM 18976 T).

UR - http://www.scopus.com/inward/record.url?scp=84874910353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874910353&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.040568-0

DO - 10.1099/ijs.0.040568-0

M3 - Article

VL - 63

SP - 1073

EP - 1082

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - PART3

ER -