Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously

Yuki Abe, Tadahiro Hasegawa, Mitsuo Yamasita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ELISA (Enzyme-Linked Immuno Sorbent Assay) chip to detect multiple allergic proteins simultaneously was developed successfully. This chip consists of multiple nitrocellulose membrane with 1mm diameter that immobilized several kinds of allergic proteins with 50ng amount only is fabricated using the multi-layer lithography. The experimental results demonstrated that 1) the specific allergen protein, and 2) the quantitative change of the antigen-antibody reaction depending on amount of antibody were able to be detected without contamination. The detection procedure that is only to inject antibodies in order into the chip is simple. The time for the whole detection procedure is short with 16 min. This ELISA chip that can specify the allergen protein that causes the patient's allergic disease is useful for the hyposensitization therapy.

Original languageEnglish
Title of host publicationProceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages675-678
Number of pages4
ISBN (Electronic)9781538636152
DOIs
Publication statusPublished - 2019 Apr 25
Event2019 IEEE/SICE International Symposium on System Integration, SII 2019 - Paris, France
Duration: 2019 Jan 142019 Jan 16

Publication series

NameProceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019

Conference

Conference2019 IEEE/SICE International Symposium on System Integration, SII 2019
CountryFrance
CityParis
Period19/1/1419/1/16

Fingerprint

sorbents
Sorbents
enzymes
Assays
Enzymes
antibodies
chips
Allergens
proteins
Proteins
Antibodies
allergic diseases
Antigen-antibody reactions
cellulose nitrate
Nitrocellulose
antigens
Lithography
therapy
contamination
Contamination

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Cite this

Abe, Y., Hasegawa, T., & Yamasita, M. (2019). Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. In Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019 (pp. 675-678). [8700438] (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SII.2019.8700438

Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. / Abe, Yuki; Hasegawa, Tadahiro; Yamasita, Mitsuo.

Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 675-678 8700438 (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abe, Y, Hasegawa, T & Yamasita, M 2019, Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. in Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019., 8700438, Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019, Institute of Electrical and Electronics Engineers Inc., pp. 675-678, 2019 IEEE/SICE International Symposium on System Integration, SII 2019, Paris, France, 19/1/14. https://doi.org/10.1109/SII.2019.8700438
Abe Y, Hasegawa T, Yamasita M. Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. In Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 675-678. 8700438. (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019). https://doi.org/10.1109/SII.2019.8700438
Abe, Yuki ; Hasegawa, Tadahiro ; Yamasita, Mitsuo. / Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 675-678 (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019).
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