Microfluidic cell culture system with on-chip hypoxic conditioning

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have demonstrated a portable microfluidic cell culture system with multi-gas (CO2 and O2) incubation which we can cultivate under hypoxia without bulky peripheral apparatus such as gas tanks, regulators, and flow controllers. The system contains a chip of 26 mm × 48 mm which is capable to diffuse CO2 and absorb O2 through a gas-permeable wall of nested media reservoir. The media was water-jacketed with aqueous solution containing 0.8 M sodium bicarbonate as CO2 supply and 1M sodium ascorbate as oxygen scavenger. The partial CO2 pressure (pCO2) in media reservoir stabilized at least 10.2% ± 0.11% for at least 72 hours. The partial O2 pressure (pO2) in the media reservoir decreased to 4.2%. Portable on-chip hypoxic culture of SV40-T2 cells for 72 h was also demonstrated.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages4474-4477
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka
Duration: 2013 Jul 32013 Jul 7

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CityOsaka
Period13/7/313/7/7

Fingerprint

Cell culture
Microfluidics
Partial pressure
Gases
Sodium bicarbonate
Sodium
Controllers
Oxygen
Water

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Takano, A., Tanaka, M., & Futai, N. (2013). Microfluidic cell culture system with on-chip hypoxic conditioning. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 4474-4477). [6610540] https://doi.org/10.1109/EMBC.2013.6610540

Microfluidic cell culture system with on-chip hypoxic conditioning. / Takano, Atsushi; Tanaka, Masato; Futai, Nobuyuki.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 4474-4477 6610540.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takano, A, Tanaka, M & Futai, N 2013, Microfluidic cell culture system with on-chip hypoxic conditioning. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6610540, pp. 4474-4477, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, 13/7/3. https://doi.org/10.1109/EMBC.2013.6610540
Takano A, Tanaka M, Futai N. Microfluidic cell culture system with on-chip hypoxic conditioning. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 4474-4477. 6610540 https://doi.org/10.1109/EMBC.2013.6610540
Takano, Atsushi ; Tanaka, Masato ; Futai, Nobuyuki. / Microfluidic cell culture system with on-chip hypoxic conditioning. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. pp. 4474-4477
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