Microfluidic culture of primary neurons with on-chip hypoxic conditioning

Atsushi Takano, Saori Inomata, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a pocket-sized microfluidic cell culture chip that enables on-chip multi-gas (CO2 and O2) control without any feedback control or external gas supply. Elevated CO2 levels (∼7%) and hypoxic conditions (O2 levels, ∼5%) were simultaneously achieved on the chip by maintaining the bottom of the chip at 37 °C and filling an adjacent reservoir with a bicarbonate/carbonate/ascorbate solution. We have also successfully cultured primary chick embryo neurons onchip under hypoxic conditions for 3 days. The neurons cultured under hypoxia showed inhibited neurite outgrowth.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages419-421
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

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Keywords

  • Hypoxic conditioning
  • Long-term culture
  • On-chip multi-gas incubation
  • Primary neuron

ASJC Scopus subject areas

  • Bioengineering

Cite this

Takano, A., Inomata, S., Tanaka, M., & Futai, N. (2013). Microfluidic culture of primary neurons with on-chip hypoxic conditioning. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 419-421). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.