Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave

Atsushi Takano, Tasuku Kon, Yasubumi Furuya, Katsumi Mochitate, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a poly(dimethylsiloxane) (PDMS)-made microfluidic long-term cell culture platform having two cell culture wells with piezoelectric substrate bottom on which shear horizontal surface acoustic wave (SH-SAW) propagates. The SH-SAW propagates on the cells could be used to assay cell-cell and/or cell-surface interaction. The platform also enables optical observation of the cells and acquisition of SH-SAW signals simultaneously. The combination of the on-chip CO2 incubation and SH-SAW-based cell monitoring system successfully detected a long-term change in cell-cell or cell-substrate interaction induced by hydrogen peroxide (H2O2).

Original languageEnglish
Title of host publication2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PublisherIEEE Computer Society
ISBN (Print)9781479915293
DOIs
Publication statusPublished - 2013
Event2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya
Duration: 2013 Nov 102013 Nov 13

Other

Other2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
CityNagoya
Period13/11/1013/11/13

Fingerprint

Cell culture
Microfluidics
Surface waves
Acoustic waves
Monitoring
Substrates
Polydimethylsiloxane
Hydrogen peroxide
Assays

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Takano, A., Kon, T., Furuya, Y., Mochitate, K., Tanaka, M., & Futai, N. (2013). Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 [6710472] IEEE Computer Society. https://doi.org/10.1109/MHS.2013.6710472

Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave. / Takano, Atsushi; Kon, Tasuku; Furuya, Yasubumi; Mochitate, Katsumi; Tanaka, Masato; Futai, Nobuyuki.

2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013. 6710472.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takano, A, Kon, T, Furuya, Y, Mochitate, K, Tanaka, M & Futai, N 2013, Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave. in 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013., 6710472, IEEE Computer Society, 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013, Nagoya, 13/11/10. https://doi.org/10.1109/MHS.2013.6710472
Takano A, Kon T, Furuya Y, Mochitate K, Tanaka M, Futai N. Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society. 2013. 6710472 https://doi.org/10.1109/MHS.2013.6710472
Takano, Atsushi ; Kon, Tasuku ; Furuya, Yasubumi ; Mochitate, Katsumi ; Tanaka, Masato ; Futai, Nobuyuki. / Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave. 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013.
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