Molecular cloning and characterization of the srdBCA operon, encoding the respiratory selenate reductase complex, from the selenate-reducing bacterium Bacillus selenatarsenatis SF-1

Masashi Kuroda, Mitsuo Yamashita, Emiko Miwa, Kanako Imao, Noriyuki Fujimoto, Hisayo Ono, Kouta Nagano, Kazunari Sei, Michihiko Ike

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Abstract

Previously, we isolated a selenate- and arsenate-reducing bacterium, designated strain SF-1, from seleniumcontaminated sediment and identified it as a novel species, Bacillus selenatarsenatis. B. selenatarsenatis strain SF-1 independently reduces selenate to selenite, arsenate to arsenite, and nitrate to nitrite by anaerobic respiration. To identify the genes involved in selenate reduction, 17 selenate reduction-defective mutant strains were isolated from a mutant library generated by random insertion of transposon Tn916. Tn916 was inserted into the same genome position in eight mutants, and the representative strain SF-1AM4 did not reduce selenate but did reduce nitrate and arsenate to the same extent as the wild-type strain. The disrupted gene was located in an operon composed of three genes designated srdBCA, which were predicted to encode a putative oxidoreductase complex by the BLASTX program. The plasmid vector pGEMsrdBCA, containing the srdBCA operon with its own promoter, conferred the phenotype of selenate reduction in Escherichia coli DH5α, although E. coli strains containing plasmids lacking any one or two of the open reading frames from srdBCA did not exhibit the selenate-reducing phenotype. Domain structure analysis of the deduced amino acid sequence revealed that SrdBCA had typical features of membrane-bound and molybdopterin-containing oxidoreductases. It was therefore proposed that the srdBCA operon encoded a respiratory selenate reductase complex. This is the first report of genes encoding selenate reductase in Gram-positive bacteria.

Original languageEnglish
Pages (from-to)2141-2148
Number of pages8
JournalJournal of Bacteriology
Volume193
Issue number9
DOIs
Publication statusPublished - 2011 May

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Molecular cloning and characterization of the srdBCA operon, encoding the respiratory selenate reductase complex, from the selenate-reducing bacterium Bacillus selenatarsenatis SF-1. / Kuroda, Masashi; Yamashita, Mitsuo; Miwa, Emiko; Imao, Kanako; Fujimoto, Noriyuki; Ono, Hisayo; Nagano, Kouta; Sei, Kazunari; Ike, Michihiko.

In: Journal of Bacteriology, Vol. 193, No. 9, 05.2011, p. 2141-2148.

Research output: Contribution to journalArticle

Kuroda, Masashi ; Yamashita, Mitsuo ; Miwa, Emiko ; Imao, Kanako ; Fujimoto, Noriyuki ; Ono, Hisayo ; Nagano, Kouta ; Sei, Kazunari ; Ike, Michihiko. / Molecular cloning and characterization of the srdBCA operon, encoding the respiratory selenate reductase complex, from the selenate-reducing bacterium Bacillus selenatarsenatis SF-1. In: Journal of Bacteriology. 2011 ; Vol. 193, No. 9. pp. 2141-2148.
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abstract = "Previously, we isolated a selenate- and arsenate-reducing bacterium, designated strain SF-1, from seleniumcontaminated sediment and identified it as a novel species, Bacillus selenatarsenatis. B. selenatarsenatis strain SF-1 independently reduces selenate to selenite, arsenate to arsenite, and nitrate to nitrite by anaerobic respiration. To identify the genes involved in selenate reduction, 17 selenate reduction-defective mutant strains were isolated from a mutant library generated by random insertion of transposon Tn916. Tn916 was inserted into the same genome position in eight mutants, and the representative strain SF-1AM4 did not reduce selenate but did reduce nitrate and arsenate to the same extent as the wild-type strain. The disrupted gene was located in an operon composed of three genes designated srdBCA, which were predicted to encode a putative oxidoreductase complex by the BLASTX program. The plasmid vector pGEMsrdBCA, containing the srdBCA operon with its own promoter, conferred the phenotype of selenate reduction in Escherichia coli DH5α, although E. coli strains containing plasmids lacking any one or two of the open reading frames from srdBCA did not exhibit the selenate-reducing phenotype. Domain structure analysis of the deduced amino acid sequence revealed that SrdBCA had typical features of membrane-bound and molybdopterin-containing oxidoreductases. It was therefore proposed that the srdBCA operon encoded a respiratory selenate reductase complex. This is the first report of genes encoding selenate reductase in Gram-positive bacteria.",
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