Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Hiromasa Ishii, Sadakazu Aiso

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1445
Issue number1
DOIs
Publication statusPublished - 1999 Apr 14
Externally publishedYes

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Keywords

  • Apoptosis
  • Embryo
  • Kruppel
  • Requiem
  • Zinc-finger

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

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