Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes

Yuji Hiraki, Hideho Tanaka, Hiroyuki Inoue, Jun Kondo, Akihito Kamizono, Fujio Suzuki

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage.

Original languageEnglish
Pages (from-to)971-977
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number3
DOIs
Publication statusPublished - 1991 Mar 29
Externally publishedYes

Fingerprint

Cloning
Cartilage
Glycosylation
Fibroblast Growth Factor 2
Bioactivity
Glycoproteins
Complementary DNA
Tissue
Amino Acids
Messenger RNA
DNA
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes. / Hiraki, Yuji; Tanaka, Hideho; Inoue, Hiroyuki; Kondo, Jun; Kamizono, Akihito; Suzuki, Fujio.

In: Biochemical and Biophysical Research Communications, Vol. 175, No. 3, 29.03.1991, p. 971-977.

Research output: Contribution to journalArticle

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