Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes

Yuji Hiraki, Hideho Tanaka, Hiroyuki Inoue, Jun Kondo, Akihito Kamizono, Fujio Suzuki

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage.

Original languageEnglish
Pages (from-to)971-977
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number3
DOIs
Publication statusPublished - 1991 Mar 29

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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