Molecular design of novel metal-binding oligomeric human metallothioneins

S. H. Hong, M. Gohya, H. Ono, H. Murakami, M. Yamashita, N. Hirayama, Y. Murooka

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22 Citations (Scopus)

Abstract

Genes for dimeric and tetrameric human metallothionein (hMT) were designed and successfully overexpressed in Escherichia coli to generate functional oligomeric hMTs. An hMT synthesized with prokaryotic codons, a linker encoding a gly-gly-gly tripeptide, and Met-deficient hMT-II was ligated to create a dimeric hMT, from which a tetrameric hMT was then constructed. The increased molecular size of the constructs resulted in improved stability and productivity in E. coli. The oligomeric proteins formed inclusion bodies which were dissolved with dithiothreitol, and the purified apo-metallothioneins were reconstituted with Cd or Zn ions in a reducing condition. The oligomeric hMT proteins incubated with Cd ions showed a typical Cd-thiolate absorbance peak at 245-255 nm. The dimeric and tetrameric hMT proteins exhibited both Cd and Zn binding activities that were respectively two and four times higher than those of the hMT-II monomer protein. These novel oligomeric hMTs may be useful in bioremediation for heavy metals.

Original languageEnglish
Pages (from-to)84-89
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume54
Issue number1
DOIs
Publication statusPublished - 2000 Aug 9

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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