Monitoring survival of phenanthrene-utilizing Sphingobium sp. P2 in soil microcosms using green fluorescent protein as a marker

Nattachai Kengpipat, Kenichi Iwata, Toshio Omori, Onruthai Pinyakong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phenanthrene is a polycyclic aromatic hydrocarbon which belongs to a class of toxic environmental pollutants that has been accumulating due to a variety of anthropogenic activities. We attempted to use Sphingobium sp. P2, which is capable of utilizing phenanthrene as its sole source of carbon and energy, in bioaugmentation treatments. For successful bioremediation, monitoring the survival and metabolic activity of bioaugmented bacteria are required. This study aimed to use green fluorescent protein as a marker to estimate the survival of Sphingobium sp. P2 in bioaugmented soil microcosms. The gfp was integrated via Tn5 transposition into the chromosome of strain P2. The gfp-inserted strains were identified by green fluorescence emission under UV light. The gfp was stably maintained in strain P2, and the strain still retained the ability to use phenanthrene as a sole carbon and energy source. Soil microcosm experiments revealed that survival of the strain P2-gfp and its phenanthrene-degrading capacity may depend on indigenous microorganisms and nutrients in the soils. The strain P2-gfp can be used to evaluate the success of bioaugmentation.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalScienceAsia
Volume36
Issue number1
DOIs
Publication statusPublished - 2010 Mar
Externally publishedYes

Fingerprint

phenanthrene
microcosm
protein
monitoring
soil
carbon
bioremediation
chromosome
PAH
fluorescence
human activity
microorganism
bacterium
marker
pollutant
nutrient
energy
experiment

Keywords

  • Bioaugmentation
  • Biodegradation
  • Bioremediation
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • General

Cite this

Monitoring survival of phenanthrene-utilizing Sphingobium sp. P2 in soil microcosms using green fluorescent protein as a marker. / Kengpipat, Nattachai; Iwata, Kenichi; Omori, Toshio; Pinyakong, Onruthai.

In: ScienceAsia, Vol. 36, No. 1, 03.2010, p. 76-80.

Research output: Contribution to journalArticle

Kengpipat, Nattachai ; Iwata, Kenichi ; Omori, Toshio ; Pinyakong, Onruthai. / Monitoring survival of phenanthrene-utilizing Sphingobium sp. P2 in soil microcosms using green fluorescent protein as a marker. In: ScienceAsia. 2010 ; Vol. 36, No. 1. pp. 76-80.
@article{0ac75f2bac564275b0a7e1c80ca0a4e9,
title = "Monitoring survival of phenanthrene-utilizing Sphingobium sp. P2 in soil microcosms using green fluorescent protein as a marker",
abstract = "Phenanthrene is a polycyclic aromatic hydrocarbon which belongs to a class of toxic environmental pollutants that has been accumulating due to a variety of anthropogenic activities. We attempted to use Sphingobium sp. P2, which is capable of utilizing phenanthrene as its sole source of carbon and energy, in bioaugmentation treatments. For successful bioremediation, monitoring the survival and metabolic activity of bioaugmented bacteria are required. This study aimed to use green fluorescent protein as a marker to estimate the survival of Sphingobium sp. P2 in bioaugmented soil microcosms. The gfp was integrated via Tn5 transposition into the chromosome of strain P2. The gfp-inserted strains were identified by green fluorescence emission under UV light. The gfp was stably maintained in strain P2, and the strain still retained the ability to use phenanthrene as a sole carbon and energy source. Soil microcosm experiments revealed that survival of the strain P2-gfp and its phenanthrene-degrading capacity may depend on indigenous microorganisms and nutrients in the soils. The strain P2-gfp can be used to evaluate the success of bioaugmentation.",
keywords = "Bioaugmentation, Biodegradation, Bioremediation, Polycyclic aromatic hydrocarbons",
author = "Nattachai Kengpipat and Kenichi Iwata and Toshio Omori and Onruthai Pinyakong",
year = "2010",
month = "3",
doi = "10.2306/scienceasial 513-1874.2010.36.076",
language = "English",
volume = "36",
pages = "76--80",
journal = "ScienceAsia",
issn = "1513-1874",
publisher = "Science Society of Thailand under Royal Patronage",
number = "1",

}

TY - JOUR

T1 - Monitoring survival of phenanthrene-utilizing Sphingobium sp. P2 in soil microcosms using green fluorescent protein as a marker

AU - Kengpipat, Nattachai

AU - Iwata, Kenichi

AU - Omori, Toshio

AU - Pinyakong, Onruthai

PY - 2010/3

Y1 - 2010/3

N2 - Phenanthrene is a polycyclic aromatic hydrocarbon which belongs to a class of toxic environmental pollutants that has been accumulating due to a variety of anthropogenic activities. We attempted to use Sphingobium sp. P2, which is capable of utilizing phenanthrene as its sole source of carbon and energy, in bioaugmentation treatments. For successful bioremediation, monitoring the survival and metabolic activity of bioaugmented bacteria are required. This study aimed to use green fluorescent protein as a marker to estimate the survival of Sphingobium sp. P2 in bioaugmented soil microcosms. The gfp was integrated via Tn5 transposition into the chromosome of strain P2. The gfp-inserted strains were identified by green fluorescence emission under UV light. The gfp was stably maintained in strain P2, and the strain still retained the ability to use phenanthrene as a sole carbon and energy source. Soil microcosm experiments revealed that survival of the strain P2-gfp and its phenanthrene-degrading capacity may depend on indigenous microorganisms and nutrients in the soils. The strain P2-gfp can be used to evaluate the success of bioaugmentation.

AB - Phenanthrene is a polycyclic aromatic hydrocarbon which belongs to a class of toxic environmental pollutants that has been accumulating due to a variety of anthropogenic activities. We attempted to use Sphingobium sp. P2, which is capable of utilizing phenanthrene as its sole source of carbon and energy, in bioaugmentation treatments. For successful bioremediation, monitoring the survival and metabolic activity of bioaugmented bacteria are required. This study aimed to use green fluorescent protein as a marker to estimate the survival of Sphingobium sp. P2 in bioaugmented soil microcosms. The gfp was integrated via Tn5 transposition into the chromosome of strain P2. The gfp-inserted strains were identified by green fluorescence emission under UV light. The gfp was stably maintained in strain P2, and the strain still retained the ability to use phenanthrene as a sole carbon and energy source. Soil microcosm experiments revealed that survival of the strain P2-gfp and its phenanthrene-degrading capacity may depend on indigenous microorganisms and nutrients in the soils. The strain P2-gfp can be used to evaluate the success of bioaugmentation.

KW - Bioaugmentation

KW - Biodegradation

KW - Bioremediation

KW - Polycyclic aromatic hydrocarbons

UR - http://www.scopus.com/inward/record.url?scp=77951105066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951105066&partnerID=8YFLogxK

U2 - 10.2306/scienceasial 513-1874.2010.36.076

DO - 10.2306/scienceasial 513-1874.2010.36.076

M3 - Article

VL - 36

SP - 76

EP - 80

JO - ScienceAsia

JF - ScienceAsia

SN - 1513-1874

IS - 1

ER -