Monoclonal Antibodies to the Secretory Granule Membrane of the Rabbit Parotid Gland: Presence of a Common Antigen in Secretory Granules of Both Exocrine and Endocrine Cells

Shuji Yamashita, Sadakazu Aiso, Kenjiro Yasuda

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2 Citations (Scopus)

Abstract

We prepared monoclonal antibodies (McAbs) against secretory granule membrane of the rabbit parotid gland. Two McAbs, IgG2a (McAb-116) and IgM (McAb-64), which were directed to the glycoprotein of 55-60 Kd which contained N-linked carbohydrate, were obtained. It was suggested that this glycoprotein was an integral membrane protein and localized on the luminal side of secretory granules. The following cells were immunostained with the McAbs: a) among exocrine cells, acinar cells of the parotid and submandibular glands, chief cells of the stomach and pancreatic exocrine cells; and b) among endocrine cells, parafollicular cells of the thyroid gland, adenohypophyseal cells, enterochromaffin cells of the digestive tract, adrenal medullary cells and juxtaglomerular cells of the kindney. These findings suggested that secretory granule specific antigen (SGSA), existed commonly in the secretory granules of both exocrine and endocrine cells. Immunoelectoron microscopy revealed that SGSA was localized exclusively along the membranes of secretory granules in the parotid acinar cells, exocrine pancreatic cells and adenohypophyseal cells. In parotid gland, SGSA was also recognized on the membranes of the Golgi lamellae and vacuoles and apical cell membranes. It is suggested that SGSA may play an important role in secretory granule formation and exocytosis in secretory cells. The two McAbs may provide a useful tool for investigating the formation, translocation and recycling of secretary granule membrane proteins.

Original languageEnglish
Pages (from-to)517-534
Number of pages18
JournalActa Histochemica et Cytochemica
Volume22
Issue number5
DOIs
Publication statusPublished - 1989 Jan 1
Externally publishedYes

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Monoclonal Antibodies
Cells
Membranes
Antigens
Glycoproteins
Membrane Proteins
Cell membranes
Immunoglobulin M
Recycling
Microscopic examination
Carbohydrates

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

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title = "Monoclonal Antibodies to the Secretory Granule Membrane of the Rabbit Parotid Gland: Presence of a Common Antigen in Secretory Granules of Both Exocrine and Endocrine Cells",
abstract = "We prepared monoclonal antibodies (McAbs) against secretory granule membrane of the rabbit parotid gland. Two McAbs, IgG2a (McAb-116) and IgM (McAb-64), which were directed to the glycoprotein of 55-60 Kd which contained N-linked carbohydrate, were obtained. It was suggested that this glycoprotein was an integral membrane protein and localized on the luminal side of secretory granules. The following cells were immunostained with the McAbs: a) among exocrine cells, acinar cells of the parotid and submandibular glands, chief cells of the stomach and pancreatic exocrine cells; and b) among endocrine cells, parafollicular cells of the thyroid gland, adenohypophyseal cells, enterochromaffin cells of the digestive tract, adrenal medullary cells and juxtaglomerular cells of the kindney. These findings suggested that secretory granule specific antigen (SGSA), existed commonly in the secretory granules of both exocrine and endocrine cells. Immunoelectoron microscopy revealed that SGSA was localized exclusively along the membranes of secretory granules in the parotid acinar cells, exocrine pancreatic cells and adenohypophyseal cells. In parotid gland, SGSA was also recognized on the membranes of the Golgi lamellae and vacuoles and apical cell membranes. It is suggested that SGSA may play an important role in secretory granule formation and exocytosis in secretory cells. The two McAbs may provide a useful tool for investigating the formation, translocation and recycling of secretary granule membrane proteins.",
author = "Shuji Yamashita and Sadakazu Aiso and Kenjiro Yasuda",
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AU - Aiso, Sadakazu

AU - Yasuda, Kenjiro

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N2 - We prepared monoclonal antibodies (McAbs) against secretory granule membrane of the rabbit parotid gland. Two McAbs, IgG2a (McAb-116) and IgM (McAb-64), which were directed to the glycoprotein of 55-60 Kd which contained N-linked carbohydrate, were obtained. It was suggested that this glycoprotein was an integral membrane protein and localized on the luminal side of secretory granules. The following cells were immunostained with the McAbs: a) among exocrine cells, acinar cells of the parotid and submandibular glands, chief cells of the stomach and pancreatic exocrine cells; and b) among endocrine cells, parafollicular cells of the thyroid gland, adenohypophyseal cells, enterochromaffin cells of the digestive tract, adrenal medullary cells and juxtaglomerular cells of the kindney. These findings suggested that secretory granule specific antigen (SGSA), existed commonly in the secretory granules of both exocrine and endocrine cells. Immunoelectoron microscopy revealed that SGSA was localized exclusively along the membranes of secretory granules in the parotid acinar cells, exocrine pancreatic cells and adenohypophyseal cells. In parotid gland, SGSA was also recognized on the membranes of the Golgi lamellae and vacuoles and apical cell membranes. It is suggested that SGSA may play an important role in secretory granule formation and exocytosis in secretory cells. The two McAbs may provide a useful tool for investigating the formation, translocation and recycling of secretary granule membrane proteins.

AB - We prepared monoclonal antibodies (McAbs) against secretory granule membrane of the rabbit parotid gland. Two McAbs, IgG2a (McAb-116) and IgM (McAb-64), which were directed to the glycoprotein of 55-60 Kd which contained N-linked carbohydrate, were obtained. It was suggested that this glycoprotein was an integral membrane protein and localized on the luminal side of secretory granules. The following cells were immunostained with the McAbs: a) among exocrine cells, acinar cells of the parotid and submandibular glands, chief cells of the stomach and pancreatic exocrine cells; and b) among endocrine cells, parafollicular cells of the thyroid gland, adenohypophyseal cells, enterochromaffin cells of the digestive tract, adrenal medullary cells and juxtaglomerular cells of the kindney. These findings suggested that secretory granule specific antigen (SGSA), existed commonly in the secretory granules of both exocrine and endocrine cells. Immunoelectoron microscopy revealed that SGSA was localized exclusively along the membranes of secretory granules in the parotid acinar cells, exocrine pancreatic cells and adenohypophyseal cells. In parotid gland, SGSA was also recognized on the membranes of the Golgi lamellae and vacuoles and apical cell membranes. It is suggested that SGSA may play an important role in secretory granule formation and exocytosis in secretory cells. The two McAbs may provide a useful tool for investigating the formation, translocation and recycling of secretary granule membrane proteins.

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