Multimodal image analysis of unstained histological sections

Takuya Ushikawa, Masanobu Takahashi, Masayuki Nakano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Unstained histological sections were used for histological image analysis. Color differences among sections and discoloration which sometimes degrade the accuracy for image analysis don’t occur in unstained sections. Staining process can also be omitted. A multimodal method is proposed to analyze unstained histological sections. The feature of our multimodal method is to use the imaging methods available in an optical microscope used in dairy diagnosis. Three kinds of images, bright-field, dark-field, and phase-contrast images were used. These images could be easily captured using a commonly available optical microscope with little additional time and cost. The method was applied for cell membrane extraction of unstained hepatic histological sections. The best result was obtained when all three kinds of images were combined, showing the effectiveness of multimodal method for analyzing unstained sections. Average correct rate was almost the same as the case which has ever been obtained for HE-stained sections. These results show promising possibility of using unstained sections for histological image analysis.

Original languageEnglish
Title of host publicationIFMBE Proceedings
PublisherSpringer Verlag
Pages216-219
Number of pages4
Volume45
ISBN (Print)9783319111278
DOIs
Publication statusPublished - 2015
Event6th European Conference of the International Federation for Medical and Biological Engineering, MBEC 2014 - Dubrovnik, Croatia
Duration: 2014 Sep 72014 Sep 11

Other

Other6th European Conference of the International Federation for Medical and Biological Engineering, MBEC 2014
CountryCroatia
CityDubrovnik
Period14/9/714/9/11

Fingerprint

Image analysis
Microscopes
Discoloration
Dairies
Cell membranes
Color
Imaging techniques
Costs

Keywords

  • Cell membrane
  • Dark field
  • Histology
  • Phasecontrast
  • Unstained

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Ushikawa, T., Takahashi, M., & Nakano, M. (2015). Multimodal image analysis of unstained histological sections. In IFMBE Proceedings (Vol. 45, pp. 216-219). Springer Verlag. https://doi.org/10.1007/978-3-319-11128-5_54

Multimodal image analysis of unstained histological sections. / Ushikawa, Takuya; Takahashi, Masanobu; Nakano, Masayuki.

IFMBE Proceedings. Vol. 45 Springer Verlag, 2015. p. 216-219.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ushikawa, T, Takahashi, M & Nakano, M 2015, Multimodal image analysis of unstained histological sections. in IFMBE Proceedings. vol. 45, Springer Verlag, pp. 216-219, 6th European Conference of the International Federation for Medical and Biological Engineering, MBEC 2014, Dubrovnik, Croatia, 14/9/7. https://doi.org/10.1007/978-3-319-11128-5_54
Ushikawa T, Takahashi M, Nakano M. Multimodal image analysis of unstained histological sections. In IFMBE Proceedings. Vol. 45. Springer Verlag. 2015. p. 216-219 https://doi.org/10.1007/978-3-319-11128-5_54
Ushikawa, Takuya ; Takahashi, Masanobu ; Nakano, Masayuki. / Multimodal image analysis of unstained histological sections. IFMBE Proceedings. Vol. 45 Springer Verlag, 2015. pp. 216-219
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