Abstract
Unstained histological sections were used for histological image analysis. Color differences among sections and discoloration which sometimes degrade the accuracy for image analysis don’t occur in unstained sections. Staining process can also be omitted. A multimodal method is proposed to analyze unstained histological sections. The feature of our multimodal method is to use the imaging methods available in an optical microscope used in dairy diagnosis. Three kinds of images, bright-field, dark-field, and phase-contrast images were used. These images could be easily captured using a commonly available optical microscope with little additional time and cost. The method was applied for cell membrane extraction of unstained hepatic histological sections. The best result was obtained when all three kinds of images were combined, showing the effectiveness of multimodal method for analyzing unstained sections. Average correct rate was almost the same as the case which has ever been obtained for HE-stained sections. These results show promising possibility of using unstained sections for histological image analysis.
| Original language | English |
|---|---|
| Title of host publication | IFMBE Proceedings |
| Publisher | Springer Verlag |
| Pages | 216-219 |
| Number of pages | 4 |
| Volume | 45 |
| ISBN (Print) | 9783319111278 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | 6th European Conference of the International Federation for Medical and Biological Engineering, MBEC 2014 - Dubrovnik, Croatia Duration: 2014 Sep 7 → 2014 Sep 11 |
Other
| Other | 6th European Conference of the International Federation for Medical and Biological Engineering, MBEC 2014 |
|---|---|
| Country | Croatia |
| City | Dubrovnik |
| Period | 14/9/7 → 14/9/11 |
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Keywords
- Cell membrane
- Dark field
- Histology
- Phasecontrast
- Unstained
ASJC Scopus subject areas
- Biomedical Engineering
- Bioengineering
Cite this
Multimodal image analysis of unstained histological sections. / Ushikawa, Takuya; Takahashi, Masanobu; Nakano, Masayuki.
IFMBE Proceedings. Vol. 45 Springer Verlag, 2015. p. 216-219.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multimodal image analysis of unstained histological sections
AU - Ushikawa, Takuya
AU - Takahashi, Masanobu
AU - Nakano, Masayuki
PY - 2015
Y1 - 2015
N2 - Unstained histological sections were used for histological image analysis. Color differences among sections and discoloration which sometimes degrade the accuracy for image analysis don’t occur in unstained sections. Staining process can also be omitted. A multimodal method is proposed to analyze unstained histological sections. The feature of our multimodal method is to use the imaging methods available in an optical microscope used in dairy diagnosis. Three kinds of images, bright-field, dark-field, and phase-contrast images were used. These images could be easily captured using a commonly available optical microscope with little additional time and cost. The method was applied for cell membrane extraction of unstained hepatic histological sections. The best result was obtained when all three kinds of images were combined, showing the effectiveness of multimodal method for analyzing unstained sections. Average correct rate was almost the same as the case which has ever been obtained for HE-stained sections. These results show promising possibility of using unstained sections for histological image analysis.
AB - Unstained histological sections were used for histological image analysis. Color differences among sections and discoloration which sometimes degrade the accuracy for image analysis don’t occur in unstained sections. Staining process can also be omitted. A multimodal method is proposed to analyze unstained histological sections. The feature of our multimodal method is to use the imaging methods available in an optical microscope used in dairy diagnosis. Three kinds of images, bright-field, dark-field, and phase-contrast images were used. These images could be easily captured using a commonly available optical microscope with little additional time and cost. The method was applied for cell membrane extraction of unstained hepatic histological sections. The best result was obtained when all three kinds of images were combined, showing the effectiveness of multimodal method for analyzing unstained sections. Average correct rate was almost the same as the case which has ever been obtained for HE-stained sections. These results show promising possibility of using unstained sections for histological image analysis.
KW - Cell membrane
KW - Dark field
KW - Histology
KW - Phasecontrast
KW - Unstained
UR - http://www.scopus.com/inward/record.url?scp=84937779606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937779606&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-11128-5_54
DO - 10.1007/978-3-319-11128-5_54
M3 - Conference contribution
AN - SCOPUS:84937779606
SN - 9783319111278
VL - 45
SP - 216
EP - 219
BT - IFMBE Proceedings
PB - Springer Verlag
ER -