Multiphase-laden gas-liquid interface injection for the versatile gene transfer

H. Kuriki, S. Takasawa, M. Iwabuchi, K. Ohsumi, T. Suzuki, T. Higashiyama, S. Sakuma, F. Arai, Y. Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1173-1175
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

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Keywords

  • Micro-bubble
  • Multiphase injection

ASJC Scopus subject areas

  • Bioengineering

Cite this

Kuriki, H., Takasawa, S., Iwabuchi, M., Ohsumi, K., Suzuki, T., Higashiyama, T., Sakuma, S., Arai, F., & Yamanishi, Y. (2013). Multiphase-laden gas-liquid interface injection for the versatile gene transfer. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1173-1175). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.