Abstract
We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1173-1175 |
| Number of pages | 3 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 2013 Oct 27 → 2013 Oct 31 |
Publication series
| Name | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Volume | 2 |
Conference
| Conference | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 13/10/27 → 13/10/31 |
Keywords
- Micro-bubble
- Multiphase injection
ASJC Scopus subject areas
- Bioengineering
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Cite this
Kuriki, H., Takasawa, S., Iwabuchi, M., Ohsumi, K., Suzuki, T., Higashiyama, T., Sakuma, S., Arai, F., & Yamanishi, Y. (2013). Multiphase-laden gas-liquid interface injection for the versatile gene transfer. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1173-1175). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.