Abstract
We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1173-1175 |
| Number of pages | 3 |
| Volume | 2 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg Duration: 2013 Oct 27 → 2013 Oct 31 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| City | Freiburg |
| Period | 13/10/27 → 13/10/31 |
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Keywords
- Micro-bubble
- Multiphase injection
ASJC Scopus subject areas
- Bioengineering
Cite this
Multiphase-laden gas-liquid interface injection for the versatile gene transfer. / Kuriki, H.; Takasawa, S.; Iwabuchi, M.; Ohsumi, K.; Suzuki, T.; Higashiyama, T.; Sakuma, S.; Arai, F.; Yamanishi, Y.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 1173-1175.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multiphase-laden gas-liquid interface injection for the versatile gene transfer
AU - Kuriki, H.
AU - Takasawa, S.
AU - Iwabuchi, M.
AU - Ohsumi, K.
AU - Suzuki, T.
AU - Higashiyama, T.
AU - Sakuma, S.
AU - Arai, F.
AU - Yamanishi, Y.
PY - 2013
Y1 - 2013
N2 - We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
AB - We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.
KW - Micro-bubble
KW - Multiphase injection
UR - http://www.scopus.com/inward/record.url?scp=84907321165&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907321165&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907321165
SN - 9781632666246
VL - 2
SP - 1173
EP - 1175
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -