Abstract
We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
| Original language | English |
|---|---|
| Pages (from-to) | 1681-1683 |
| Number of pages | 3 |
| Journal | Applied Optics |
| Volume | 36 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1997 Mar 1 |
| Externally published | Yes |
Keywords
- Biological samples in water
- Collection mode
- Evanescent field
- Near-field optical microscope
- Optical feedback
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Engineering (miscellaneous)
- Electrical and Electronic Engineering