Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

M. Naya; R. Micheletto; S. Mononobe; R. Uma Maheswari; M. Ohtsu

doi:10.1364/AO.36.001681

Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

M. Naya, R. Micheletto, S. Mononobe, R. Uma Maheswari, M. Ohtsu

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.

Original language	English
Pages (from-to)	1681-1683
Number of pages	3
Journal	Applied Optics
Volume	36
Issue number	7
DOIs	https://doi.org/10.1364/AO.36.001681
Publication status	Published - 1997 Mar 1
Externally published	Yes

Keywords

Biological samples in water
Collection mode
Evanescent field
Near-field optical microscope
Optical feedback

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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abstract = "We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.",

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AU - Naya, M.

AU - Micheletto, R.

AU - Mononobe, S.

AU - Uma Maheswari, R.

AU - Ohtsu, M.

PY - 1997/3/1

Y1 - 1997/3/1

N2 - We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.

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KW - Biological samples in water

KW - Collection mode

KW - Evanescent field

KW - Near-field optical microscope

KW - Optical feedback

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Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

Abstract

Keywords

ASJC Scopus subject areas

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