Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

M. Naya; R. Micheletto; S. Mononobe; R. Uma Maheswari; M. Ohtsu

doi:10.1364/AO.36.001681

Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

M. Naya, R. Micheletto, S. Mononobe, R. Uma Maheswari, M. Ohtsu

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.

Original language	English
Pages (from-to)	1681-1683
Number of pages	3
Journal	Applied Optics
Volume	36
Issue number	7
DOIs	https://doi.org/10.1364/AO.36.001681
Publication status	Published - 1997 Mar 1

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Keywords

Biological samples in water
Collection mode
Evanescent field
Near-field optical microscope
Optical feedback

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

Cite this

Naya, M., Micheletto, R., Mononobe, S., Uma Maheswari, R., & Ohtsu, M. (1997). Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control. Applied Optics, 36(7), 1681-1683. https://doi.org/10.1364/AO.36.001681

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10.1364/AO.36.001681