Abstract
We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
| Original language | English |
|---|---|
| Pages (from-to) | 1681-1683 |
| Number of pages | 3 |
| Journal | Applied Optics |
| Volume | 36 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1997 Mar 1 |
| Externally published | Yes |
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Keywords
- Biological samples in water
- Collection mode
- Evanescent field
- Near-field optical microscope
- Optical feedback
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control. / Naya, M.; Micheletto, R.; Mononobe, S.; Umamaheswari, Rajagopalan; Ohtsu, M.
In: Applied Optics, Vol. 36, No. 7, 01.03.1997, p. 1681-1683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control
AU - Naya, M.
AU - Micheletto, R.
AU - Mononobe, S.
AU - Umamaheswari, Rajagopalan
AU - Ohtsu, M.
PY - 1997/3/1
Y1 - 1997/3/1
N2 - We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
AB - We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
KW - Biological samples in water
KW - Collection mode
KW - Evanescent field
KW - Near-field optical microscope
KW - Optical feedback
UR - http://www.scopus.com/inward/record.url?scp=0008605231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0008605231&partnerID=8YFLogxK
U2 - 10.1364/AO.36.001681
DO - 10.1364/AO.36.001681
M3 - Article
C2 - 18250854
AN - SCOPUS:0008605231
VL - 36
SP - 1681
EP - 1683
JO - Applied Optics
JF - Applied Optics
SN - 0003-6935
IS - 7
ER -