NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION.

Masato Yoshida, Atsushi Kawarada, Masahiro Shibata, Akira Kamiya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To study the transcapillary exchange of water-soluble substances, the authors tried a new method to measure the capillary permeability surface area product (PS) under a vital microscopic field. As a blue dye indicator, Cr-EDTA (MW:341, maximum light absorption at 542nm) was solved in the suffusate of a Tyrode solution (20g/dl) and stained the rabbit tenuissimus muscle for about 30 min. After changing it to a Cr-EDTA free solution, the rate of the indicator washout from tissue to capillary was recorded with a photocell placed on a VTR monitor connected to the microscope. The time constant of the clearance curve gave the reciprocal of the PS value. By this method the PS values reflect the local variations in the substance exchange rate might be obtained. The fundamental nature of the clearance curve was studied, including the effect of the local capillary flow.

Original languageEnglish
Title of host publicationJapanese Journal of Medical Electronics and Biological Engineering
PublisherJapan Soc of Medical Electronics & Biological Engineering
Pages192-193
Number of pages2
Volume22
Publication statusPublished - 1984 Apr
Externally publishedYes

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Ethylenediaminetetraacetic acid
Muscle
Photoelectric cells
Capillary flow
Light absorption
Microscopes
Dyes
Tissue
Water

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Yoshida, M., Kawarada, A., Shibata, M., & Kamiya, A. (1984). NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION. In Japanese Journal of Medical Electronics and Biological Engineering (Vol. 22, pp. 192-193). Japan Soc of Medical Electronics & Biological Engineering.

NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION. / Yoshida, Masato; Kawarada, Atsushi; Shibata, Masahiro; Kamiya, Akira.

Japanese Journal of Medical Electronics and Biological Engineering. Vol. 22 Japan Soc of Medical Electronics & Biological Engineering, 1984. p. 192-193.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yoshida, M, Kawarada, A, Shibata, M & Kamiya, A 1984, NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION. in Japanese Journal of Medical Electronics and Biological Engineering. vol. 22, Japan Soc of Medical Electronics & Biological Engineering, pp. 192-193.
Yoshida M, Kawarada A, Shibata M, Kamiya A. NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION. In Japanese Journal of Medical Electronics and Biological Engineering. Vol. 22. Japan Soc of Medical Electronics & Biological Engineering. 1984. p. 192-193
Yoshida, Masato ; Kawarada, Atsushi ; Shibata, Masahiro ; Kamiya, Akira. / NEW METHOD TO MEASURE THE CAPILLARY PERMEABILITY SURFACE AREA PRODUCT (PS) IN RABBIT SKELETAL MUSCLE UNDER VITAL MICROSCOPIC OBSERVATION. Japanese Journal of Medical Electronics and Biological Engineering. Vol. 22 Japan Soc of Medical Electronics & Biological Engineering, 1984. pp. 192-193
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