Abstract
To study the process of O2 transport to tissue, we investigated how capillary perfusion is controlled in response to changes in tissue O2 levels in skeletal muscle. Capillary red blood cell (RBC) velocity and perfused capillary recruitment were measured in rabbit tenuissimus muscle at various ambient oxygen tensions (pO2) by intravital microscopy. Both RBC velocity and capillary recruitment significantly decreased as the pO2 level of the suffusate was increased, and the relationship between capillary perfusion, calculated from the velocity and recruitment data, and the pO2 level of the suffusate clearly yielded a nonlinear correlation that fitted a sigmoidal curve. Capillary perfusion dramatically decreases or increases above or below a suffusate pO2 level of around 40 Torr, where the O2 dissociation curve of hemoglobin changes slope. These findings support the hypothesis that microvasculature possesses an intrinsic, effective flow-control mechanism by sensing the metabolic demands of tissue, intimately related to the O2 saturation of hemoglobin.
| Original language | English |
|---|---|
| Pages (from-to) | 352-355 |
| Number of pages | 4 |
| Journal | European Journal of Applied Physiology |
| Volume | 94 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 Jun |
| Externally published | Yes |
Keywords
- Capillary RBC velocity
- Capillary recruitment
- Hemoglobin dissociation curve
- Oxygen transport
- Rabbit skeletal muscle
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Physiology
- Orthopedics and Sports Medicine
- Physical Therapy, Sports Therapy and Rehabilitation
Cite this
Nonlinear regulation of capillary perfusion in relation to ambient pO2 changes in skeletal muscle. / Shibata, Masahiro; Ichioka, Shigeru; Ando, Joji; Togawa, Tatsuo; Kamiya, Akira.
In: European Journal of Applied Physiology, Vol. 94, No. 3, 06.2005, p. 352-355.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nonlinear regulation of capillary perfusion in relation to ambient pO2 changes in skeletal muscle
AU - Shibata, Masahiro
AU - Ichioka, Shigeru
AU - Ando, Joji
AU - Togawa, Tatsuo
AU - Kamiya, Akira
PY - 2005/6
Y1 - 2005/6
N2 - To study the process of O2 transport to tissue, we investigated how capillary perfusion is controlled in response to changes in tissue O2 levels in skeletal muscle. Capillary red blood cell (RBC) velocity and perfused capillary recruitment were measured in rabbit tenuissimus muscle at various ambient oxygen tensions (pO2) by intravital microscopy. Both RBC velocity and capillary recruitment significantly decreased as the pO2 level of the suffusate was increased, and the relationship between capillary perfusion, calculated from the velocity and recruitment data, and the pO2 level of the suffusate clearly yielded a nonlinear correlation that fitted a sigmoidal curve. Capillary perfusion dramatically decreases or increases above or below a suffusate pO2 level of around 40 Torr, where the O2 dissociation curve of hemoglobin changes slope. These findings support the hypothesis that microvasculature possesses an intrinsic, effective flow-control mechanism by sensing the metabolic demands of tissue, intimately related to the O2 saturation of hemoglobin.
AB - To study the process of O2 transport to tissue, we investigated how capillary perfusion is controlled in response to changes in tissue O2 levels in skeletal muscle. Capillary red blood cell (RBC) velocity and perfused capillary recruitment were measured in rabbit tenuissimus muscle at various ambient oxygen tensions (pO2) by intravital microscopy. Both RBC velocity and capillary recruitment significantly decreased as the pO2 level of the suffusate was increased, and the relationship between capillary perfusion, calculated from the velocity and recruitment data, and the pO2 level of the suffusate clearly yielded a nonlinear correlation that fitted a sigmoidal curve. Capillary perfusion dramatically decreases or increases above or below a suffusate pO2 level of around 40 Torr, where the O2 dissociation curve of hemoglobin changes slope. These findings support the hypothesis that microvasculature possesses an intrinsic, effective flow-control mechanism by sensing the metabolic demands of tissue, intimately related to the O2 saturation of hemoglobin.
KW - Capillary RBC velocity
KW - Capillary recruitment
KW - Hemoglobin dissociation curve
KW - Oxygen transport
KW - Rabbit skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=20344397668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20344397668&partnerID=8YFLogxK
U2 - 10.1007/s00421-005-1315-6
DO - 10.1007/s00421-005-1315-6
M3 - Article
VL - 94
SP - 352
EP - 355
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
SN - 1439-6319
IS - 3
ER -