TY - JOUR
T1 - Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo
AU - Maheswari, R. Uma
AU - Takaoka, H.
AU - Kadono, H.
AU - Homma, R.
AU - Tanifuji, M.
N1 - Funding Information:
We would like to thank Dr Hongbin Li for his time in preparation of the anatomical sections. Supported in part by Monbukakgakusho grant with grant No. 11750048.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/3/30
Y1 - 2003/3/30
N2 - Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.
AB - Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.
KW - Depth resolved functional profile
KW - Functional optical coherence tomography
KW - Optical coherence tomography
KW - Optical intrinsic signal imaging
KW - Primary visual cortex
KW - Scattering change
KW - Visualization of neural activity
KW - Volumetric functional imaging
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U2 - 10.1016/S0165-0270(02)00370-9
DO - 10.1016/S0165-0270(02)00370-9
M3 - Article
C2 - 12648767
AN - SCOPUS:0037473238
SN - 0165-0270
VL - 124
SP - 83
EP - 92
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -