Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo

Rajagopalan Umamaheswari, H. Takaoka, H. Kadono, R. Homma, M. Tanifuji

Research output: Contribution to journal › Article

73 Citations (Scopus)

Abstract

Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalJournal of Neuroscience Methods
Volume124
Issue number1
DOIs
Publication statusPublished - 2003 Mar 30
Externally publishedYes

Keywords

  • Depth resolved functional profile
  • Functional optical coherence tomography
  • Optical coherence tomography
  • Optical intrinsic signal imaging
  • Primary visual cortex
  • Scattering change
  • Visualization of neural activity
  • Volumetric functional imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo. / Umamaheswari, Rajagopalan; Takaoka, H.; Kadono, H.; Homma, R.; Tanifuji, M.

In: Journal of Neuroscience Methods, Vol. 124, No. 1, 30.03.2003, p. 83-92.

Research output: Contribution to journal › Article

Umamaheswari, R, Takaoka, H, Kadono, H, Homma, R & Tanifuji, M 2003, 'Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo', Journal of Neuroscience Methods, vol. 124, no. 1, pp. 83-92. https://doi.org/10.1016/S0165-0270(02)00370-9
Umamaheswari R, Takaoka H, Kadono H, Homma R, Tanifuji M. Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo. Journal of Neuroscience Methods. 2003 Mar 30;124(1):83-92. https://doi.org/10.1016/S0165-0270(02)00370-9
Umamaheswari, Rajagopalan ; Takaoka, H. ; Kadono, H. ; Homma, R. ; Tanifuji, M. / Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo. In: Journal of Neuroscience Methods. 2003 ; Vol. 124, No. 1. pp. 83-92.
@article{ab09acc927bc40e69032cc724c0edcbe,
title = "Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo",
abstract = "Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.",
keywords = "Depth resolved functional profile, Functional optical coherence tomography, Optical coherence tomography, Optical intrinsic signal imaging, Primary visual cortex, Scattering change, Visualization of neural activity, Volumetric functional imaging",
author = "Rajagopalan Umamaheswari and H. Takaoka and H. Kadono and R. Homma and M. Tanifuji",
year = "2003",
month = "3",
day = "30",
doi = "10.1016/S0165-0270(02)00370-9",
language = "English",
volume = "124",
pages = "83--92",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo

AU - Umamaheswari, Rajagopalan

AU - Takaoka, H.

AU - Kadono, H.

AU - Homma, R.

AU - Tanifuji, M.

PY - 2003/3/30

Y1 - 2003/3/30

N2 - Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.

AB - Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.

KW - Depth resolved functional profile

KW - Functional optical coherence tomography

KW - Optical coherence tomography

KW - Optical intrinsic signal imaging

KW - Primary visual cortex

KW - Scattering change

KW - Visualization of neural activity

KW - Volumetric functional imaging

UR - http://www.scopus.com/inward/record.url?scp=0037473238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037473238&partnerID=8YFLogxK

U2 - 10.1016/S0165-0270(02)00370-9

DO - 10.1016/S0165-0270(02)00370-9

M3 - Article

VL - 124

SP - 83

EP - 92

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1

ER -

Access to Document