Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo

R. Uma Maheswari, H. Takaoka, H. Kadono, R. Homma, M. Tanifuji

Research output: Contribution to journalArticle

75 Citations (Scopus)


Mapping of the activity of brain by optical intrinsic signal imaging (OISI) provides a two-dimensional activation pattern of visual cortical areas at a resolution of a few hundred microns. However, integration of the intrinsic signal over depth results in loss of finer information about functional organization across the depth. Here, we report the first successful implementation of optical coherence tomography (OCT) at around 30 μm depth resolution to investigate cortical functions of a cat brain in vivo. This technique, named functional OCT (fOCT) provided visually evoked changes in the OCT signal. The fOCT signal shows stimulus specificity that correlates well with that of the intrinsic signals and provides depth resolved layer specific functional information.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalJournal of Neuroscience Methods
Issue number1
Publication statusPublished - 2003 Mar 30



  • Depth resolved functional profile
  • Functional optical coherence tomography
  • Optical coherence tomography
  • Optical intrinsic signal imaging
  • Primary visual cortex
  • Scattering change
  • Visualization of neural activity
  • Volumetric functional imaging

ASJC Scopus subject areas

  • Neuroscience(all)

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