Novel method for the continuous measurement of endotoxin concentration

Yasuo Yoshimi, Morifumi Yotsumoto, Kiyotaka Sakai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conventional limulus amebocyte lysate tests involved procedures to prevent contamination by atmospheric endotoxins. To address this problem, the authors have proposed a technique in which the sampling, reagent mixing, and reaction steps are carried out consecutively in a single tube. Since reagents do not come in contact with the atmosphere, the new technique promises stable determination of the concentration of endotoxins in dialysate fluid. An aqueous solution of endotoxin simulating dialysate fluid was sampled in a silicone rubber tube from a sterile infusion bag, then mixed with an indicator in the same tube. After reaction at 310 K, measurements were made of light absorbance at 405 nm and its linearity with endotoxin concentration was determined. Results showed a high degree of linearity (correlation coefficient of not less than 0.99) at endotoxin concentrations of 0-15 pg/ml. The time for the reaction was shortened to 12 min, in which case the response time was 15 min. It is suggested that this new test for determining endotoxin concentration using limulus amebocyte lysate reagent, in which all three steps - sampling, mixing, and reaction - proceed continuously in a single tube, offers higher reliability, greater ease of operation, and shorter response time than conventional tests.

Original languageEnglish
Pages (from-to)68-71
Number of pages4
JournalASAIO Journal
Volume41
Issue number1
Publication statusPublished - 1995 Jan
Externally publishedYes

Fingerprint

Endotoxins
Sampling
Fluids
Silicones
Rubber
Contamination
Dialysis Solutions
Silicone Elastomers
endotoxin binding proteins

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Novel method for the continuous measurement of endotoxin concentration. / Yoshimi, Yasuo; Yotsumoto, Morifumi; Sakai, Kiyotaka.

In: ASAIO Journal, Vol. 41, No. 1, 01.1995, p. 68-71.

Research output: Contribution to journalArticle

Yoshimi, Y, Yotsumoto, M & Sakai, K 1995, 'Novel method for the continuous measurement of endotoxin concentration', ASAIO Journal, vol. 41, no. 1, pp. 68-71.
Yoshimi, Yasuo ; Yotsumoto, Morifumi ; Sakai, Kiyotaka. / Novel method for the continuous measurement of endotoxin concentration. In: ASAIO Journal. 1995 ; Vol. 41, No. 1. pp. 68-71.
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