Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli

Hiroyuki Azakami, Mitsuo Yamashita, Jung Hyeob Roh, Hideyuki Suzuki, Hidehiko Kumagai, Yoshikatsu Murooka

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We found that the structural gene for monoamine oxidase was located at 30.9 min on the Escherichia coli chromosome. Deletion analysis showed that two amine oxidase genes are located in this region. The nucleotide sequence of one of the two genes was determined. The peptide sequence of the first 40 amino acids from the N terminus of monoamine oxidase purified from E. coli agrees with that deduced from the nucleotide sequence of the gene. The leader peptide extends over 30 amino acids. The nucleotide sequence of the gene and amino acid sequence of the predicted mature enzyme (M.W. 81,295) were highly homologous to those of the maoAK gene and monoamine oxidase from Klebsiella aerogenes, respectively. From these results and analysis of the enzyme activity, we concluded that the gene encodes for monoamine oxidase (maoAE). The tyrosyl residue, which may be converted to topa quinone in the E. coli enzyme, was located by comparison with amino acid sequences at the cofactor sites in other copper/topa quinone-containing amine oxidases.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume77
Issue number3
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Monoamine Oxidase
Nucleotides
Escherichia coli
Genes
Amino acids
Amino Acids
Amines
Oxidoreductases
Enzymes
Enzyme activity
Chromosomes
Protein Sorting Signals
Peptides
Copper

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli. / Azakami, Hiroyuki; Yamashita, Mitsuo; Roh, Jung Hyeob; Suzuki, Hideyuki; Kumagai, Hidehiko; Murooka, Yoshikatsu.

In: Journal of Fermentation and Bioengineering, Vol. 77, No. 3, 1994, p. 315-319.

Research output: Contribution to journalArticle

Azakami, Hiroyuki ; Yamashita, Mitsuo ; Roh, Jung Hyeob ; Suzuki, Hideyuki ; Kumagai, Hidehiko ; Murooka, Yoshikatsu. / Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli. In: Journal of Fermentation and Bioengineering. 1994 ; Vol. 77, No. 3. pp. 315-319.
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AU - Kumagai, Hidehiko

AU - Murooka, Yoshikatsu

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