Numerical and experimental evaluation of platelet deposition to collagen coated surface at low shear rates

Klaus Affeld, Leonid Goubergrits, Nobuo Watanabe, Ulrich Kertzscher

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Platelet deposition to collagen-coated surface under low shear conditions was investigated using an experimental model. The flow chamber was created by combining a stationary and a rotational glass plates spaced 50. μm apart. Blood filled into this space was subjected to a simple Couette flow. Both glass plates were covered with albumin to render them anti-thrombogenic. However, one spot 1×1. mm in size was covered with collagen. This spot was where the platelets deposited. The device was mounted on an inverted microscope and the platelet deposition was recorded. Platelets were dyed to render them fluorescent. The blood used was human blood from healthy volunteers. It was subjected to a range of low shear rates (below 700. 1/s) to find out how they act on platelet deposition. The results show a characteristic curve with elevated platelet deposition in the range of 150. 1/s. For the interpretation of these results a numerical model was developed. It applies the Monte Carlo method to model a random walk of platelets. This diffusive motion was superimposed on the convective motion by the Couette flow. A satisfactory match to the experimental data was achieved.

Original languageEnglish
Pages (from-to)430-436
Number of pages7
JournalJournal of Biomechanics
Volume46
Issue number2
DOIs
Publication statusPublished - 2013 Jan 18
Externally publishedYes

Keywords

  • Couette flow
  • Monte Carlo method
  • Platelet deposition
  • Shear rate

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

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