Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope

Rajagopalan Umamaheswari, Shuji Mononobe, Kenji Yoshida, Mamoru Yoshimoto, Motoichi Ohtsu

Research output: Contribution to journalConference article

Abstract

We present the observation results of a single string DNA (deoxy ribo nucleic acid) obtained by collection mode near-field optical microscope (NOM) operated under constant distance mode with the optical near field intensity as the feedback signal. The observed width of the narrowest string is 4 nm. To our knowledge, this is not only the successful optical observation of a single string DNA by purely optical means but also under such high resolving capability. These are attributed to the special care taken during the sample preparation to avoid unwanted scattered light and to the probe which were specially designed to enhance the local near field interaction. All these factors make the probe picks up efficiently the high spatial frequency components of the scattered near field at the same time rejecting effectively the lower spatial frequency components.

Original languageEnglish
Pages (from-to)446-451
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3729
Publication statusPublished - 1999 Jan 1
Externally publishedYes
EventProceedings of the 1998 International Conference on Optics and Optoelectronics - Silver Jubilee Symposium of the Optical Society of India - Dehradum, India
Duration: 1998 Dec 91998 Dec 12

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Aluminum Oxide
Nucleic acids
nucleic acids
optical microscopes
Sapphire
Nucleic Acids
near fields
sapphire
Microscopes
strings
Substrates
Feedback
probes
preparation
interactions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope. / Umamaheswari, Rajagopalan; Mononobe, Shuji; Yoshida, Kenji; Yoshimoto, Mamoru; Ohtsu, Motoichi.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3729, 01.01.1999, p. 446-451.

Research output: Contribution to journalConference article

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