Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy

Izabela Irena Rzeznicka, George W H Wurpel, Mischa Bonn, Michael A. van der Horst, Klaas J. Hellingwerf, Soichiro Matsunaga, Taro Yamada, Maki Kawai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalChemical Physics Letters
Volume472
Issue number1-3
DOIs
Publication statusPublished - 2009 Apr 6
Externally publishedYes

Fingerprint

Scanning tunneling microscopy
scanning tunneling microscopy
proteins
Fluorescence
Photometry
fluorescence
Proteins
photometry
3-Mercaptopropionic Acid
Carbodiimides
acids
immobilization
Amides
amides
Adsorption
adsorption

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

Cite this

Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy. / Rzeznicka, Izabela Irena; Wurpel, George W H; Bonn, Mischa; van der Horst, Michael A.; Hellingwerf, Klaas J.; Matsunaga, Soichiro; Yamada, Taro; Kawai, Maki.

In: Chemical Physics Letters, Vol. 472, No. 1-3, 06.04.2009, p. 113-117.

Research output: Contribution to journalArticle

Rzeznicka, II, Wurpel, GWH, Bonn, M, van der Horst, MA, Hellingwerf, KJ, Matsunaga, S, Yamada, T & Kawai, M 2009, 'Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy', Chemical Physics Letters, vol. 472, no. 1-3, pp. 113-117. https://doi.org/10.1016/j.cplett.2009.02.067
Rzeznicka, Izabela Irena ; Wurpel, George W H ; Bonn, Mischa ; van der Horst, Michael A. ; Hellingwerf, Klaas J. ; Matsunaga, Soichiro ; Yamada, Taro ; Kawai, Maki. / Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy. In: Chemical Physics Letters. 2009 ; Vol. 472, No. 1-3. pp. 113-117.
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