Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy

Izabela I. Rzeźnicka, George W.H. Wurpel, Mischa Bonn, Michael A. van der Horst, Klaas J. Hellingwerf, Soichiro Matsunaga, Taro Yamada, Maki Kawai

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Abstract

The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalChemical Physics Letters
Volume472
Issue number1-3
DOIs
Publication statusPublished - 2009 Apr 6

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ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Rzeźnicka, I. I., Wurpel, G. W. H., Bonn, M., van der Horst, M. A., Hellingwerf, K. J., Matsunaga, S., Yamada, T., & Kawai, M. (2009). Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy. Chemical Physics Letters, 472(1-3), 113-117. https://doi.org/10.1016/j.cplett.2009.02.067