Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

Rajagopalan Umamaheswari, Shuji Mononobe, Hitoshi Tatsumi, Yoshifumi Katayama, Motoichi Ohtsu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalOptical Review
Volume3
Issue number6 B
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Fingerprint

optical microscopes
neurons
feedback control
near fields
illumination
discrimination
sensitivity
air
liquids
diffraction

Keywords

  • Evanescent feedback
  • Illumination mode
  • Nanometric protruding tip
  • Near-field optical microscope
  • Sample feature dependent discrimination sensitivity

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control. / Umamaheswari, Rajagopalan; Mononobe, Shuji; Tatsumi, Hitoshi; Katayama, Yoshifumi; Ohtsu, Motoichi.

In: Optical Review, Vol. 3, No. 6 B, 01.01.1996, p. 463-467.

Research output: Contribution to journalArticle

Umamaheswari, Rajagopalan ; Mononobe, Shuji ; Tatsumi, Hitoshi ; Katayama, Yoshifumi ; Ohtsu, Motoichi. / Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control. In: Optical Review. 1996 ; Vol. 3, No. 6 B. pp. 463-467.
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