Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

Rajagopalan Uma Maheswari; Shuji Mononobe; Hitoshi Tatsumi; Yoshifumi Katayama; Motoichi Ohtsu

doi:10.1007/BF02932048

Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

Rajagopalan Uma Maheswari, Shuji Mononobe, Hitoshi Tatsumi, Yoshifumi Katayama, Motoichi Ohtsu

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.

Original language	English
Pages (from-to)	463-467
Number of pages	5
Journal	Optical Review
Volume	3
Issue number	6
DOIs	https://doi.org/10.1007/BF02932048
Publication status	Published - 1996
Externally published	Yes

Keywords

Evanescent feedback
Illumination mode
Nanometric protruding tip
Near-field optical microscope
Sample feature dependent discrimination sensitivity

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1007/BF02932048

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title = "Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control",

abstract = "We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.",

keywords = "Evanescent feedback, Illumination mode, Nanometric protruding tip, Near-field optical microscope, Sample feature dependent discrimination sensitivity",

author = "{Uma Maheswari}, Rajagopalan and Shuji Mononobe and Hitoshi Tatsumi and Yoshifumi Katayama and Motoichi Ohtsu",

year = "1996",

doi = "10.1007/BF02932048",

language = "English",

volume = "3",

pages = "463--467",

journal = "Optical Review",

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T1 - Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

AU - Uma Maheswari, Rajagopalan

AU - Mononobe, Shuji

AU - Tatsumi, Hitoshi

AU - Katayama, Yoshifumi

AU - Ohtsu, Motoichi

PY - 1996

Y1 - 1996

N2 - We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.

AB - We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.

KW - Evanescent feedback

KW - Illumination mode

KW - Nanometric protruding tip

KW - Near-field optical microscope

KW - Sample feature dependent discrimination sensitivity

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JO - Optical Review

JF - Optical Review

SN - 1340-6000

IS - 6

ER -

Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

Abstract

Keywords

ASJC Scopus subject areas

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