Abstract
We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.
| Original language | English |
|---|---|
| Pages (from-to) | 463-467 |
| Number of pages | 5 |
| Journal | Optical Review |
| Volume | 3 |
| Issue number | 6 B |
| DOIs | |
| Publication status | Published - 1996 Jan 1 |
| Externally published | Yes |
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Keywords
- Evanescent feedback
- Illumination mode
- Nanometric protruding tip
- Near-field optical microscope
- Sample feature dependent discrimination sensitivity
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control. / Umamaheswari, Rajagopalan; Mononobe, Shuji; Tatsumi, Hitoshi; Katayama, Yoshifumi; Ohtsu, Motoichi.
In: Optical Review, Vol. 3, No. 6 B, 01.01.1996, p. 463-467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control
AU - Umamaheswari, Rajagopalan
AU - Mononobe, Shuji
AU - Tatsumi, Hitoshi
AU - Katayama, Yoshifumi
AU - Ohtsu, Motoichi
PY - 1996/1/1
Y1 - 1996/1/1
N2 - We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.
AB - We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.
KW - Evanescent feedback
KW - Illumination mode
KW - Nanometric protruding tip
KW - Near-field optical microscope
KW - Sample feature dependent discrimination sensitivity
UR - http://www.scopus.com/inward/record.url?scp=2342656599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342656599&partnerID=8YFLogxK
U2 - 10.1007/BF02932048
DO - 10.1007/BF02932048
M3 - Article
AN - SCOPUS:2342656599
VL - 3
SP - 463
EP - 467
JO - Optical Review
JF - Optical Review
SN - 1340-6000
IS - 6 B
ER -