On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

Original languageEnglish
Article number5299057
Pages (from-to)312-317
Number of pages6
JournalIEEE Transactions on Nanobioscience
Volume8
Issue number4
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • Biological cells
  • Microelectrodes
  • Photolithography
  • Temperature control

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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