On-chip CO 2 incubation for pocket-sized microfluidic cell culture

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have developed an on-chip CO 2 incubation system based on mass/heat transfer from aqueous solutions of bicarbonate source to cell culture media through a permeable poly(dimethylsiloxane) (PDMS) wall. Heating a carbonate-buffered bicarbonate solution successfully regulated CO 2 generation without any feedback control. Because a microfluidic cell culture chip with the incubation system does not require an external chamber or gas supply, the entire microfluidic cell culture setup becomes pocket sized. Using 5 ml of 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water jacket, the chip maintained the temperature, osmolality, and pH of 750 μl cell culture medium within physiological levels when the chip was placed on a 37°C surface. The osmolality shift and pCO 2 of the media reservoir stabilized within <5 mmol/kg and 5.0 ± 1.0% over at least 9 days. The incubation capabilities were demonstrated through microfluidic culture of COS-7 epithelial cells under an inverted microscope for 17 days.

Original languageEnglish
Pages (from-to)907-915
Number of pages9
JournalMicrofluidics and Nanofluidics
Volume12
Issue number6
DOIs
Publication statusPublished - 2012 May
Externally publishedYes

Fingerprint

Carbon Monoxide
Cell culture
Microfluidics
chips
carbonates
Bicarbonates
culture media
Culture Media
Carbonates
Sodium bicarbonate
Sodium Bicarbonate
Gas supply
sodium carbonates
Polydimethylsiloxane
jackets
Feedback control
Microscopes
feedback control
Sodium
Heat transfer

Keywords

  • Braille device
  • Cell culture
  • Microfluidic
  • On-chip CO incubation

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

On-chip CO 2 incubation for pocket-sized microfluidic cell culture. / Takano, Atsushi; Tanaka, Masato; Futai, Nobuyuki.

In: Microfluidics and Nanofluidics, Vol. 12, No. 6, 05.2012, p. 907-915.

Research output: Contribution to journalArticle

Takano, Atsushi ; Tanaka, Masato ; Futai, Nobuyuki. / On-chip CO 2 incubation for pocket-sized microfluidic cell culture. In: Microfluidics and Nanofluidics. 2012 ; Vol. 12, No. 6. pp. 907-915.
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