On-chip Co2 incubation of microfluidic cell culture devices by humidity/gas exchange through poly (dimethylsiloxane) membrane

Nobuyuki Futai, Atsushi Takano, Mika Miyashita, Masato Tanaka

Research output: Contribution to journalArticle


We demonstrated an on-chip COO2 incubation system suitable for microfluidic cell culture that does not need an external chamber or gas supply. A nested pair of reservoirs for liquids insulated by gas/vaporpermeable poly (dimethylsiloxane) (PDMS) enabled COO2 incubation of media stored in the inner reservoir. This was achieved by only flowing heated sodium bicarbonate solution through the outer reservoir. The stabilization capability of temperature and pH was evaluated in situ with a miniature thermocouple and by absorption spectroscopy of phenol red, respectively. The temperature and pH were stabilized within the range of 37.0 ± 0.2°C and pH7.3 ±0.2 over at least 24 hours. This incubation capability was demonstrated through low-density microfluidic culture of CV-1 epithelial cells under an inverted microscope for three days.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalTransactions of Japanese Society for Medical and Biological Engineering
Issue number6
Publication statusPublished - 2009
Externally publishedYes



  • Cell culture
  • Co incubator
  • Microfluidic device
  • PDMS

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this