On-chip incubation system for long-term microfluidic cell culture

Atsushi Takano, Tomohisa Ogawa, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within < 3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages8404-8407
Number of pages4
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA
Duration: 2011 Aug 302011 Sep 3

Other

Other33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
CityBoston, MA
Period11/8/3011/9/3

Fingerprint

Cell culture
Microfluidics
Polydimethylsiloxane
Sodium bicarbonate
Gas supply
Water
Carbonates
Microscopes
Sodium
Glass
System-on-chip

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Takano, A., Ogawa, T., Tanaka, M., & Futai, N. (2011). On-chip incubation system for long-term microfluidic cell culture. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 8404-8407). [6092073] https://doi.org/10.1109/IEMBS.2011.6092073

On-chip incubation system for long-term microfluidic cell culture. / Takano, Atsushi; Ogawa, Tomohisa; Tanaka, Masato; Futai, Nobuyuki.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. p. 8404-8407 6092073.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takano, A, Ogawa, T, Tanaka, M & Futai, N 2011, On-chip incubation system for long-term microfluidic cell culture. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6092073, pp. 8404-8407, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011, Boston, MA, 11/8/30. https://doi.org/10.1109/IEMBS.2011.6092073
Takano A, Ogawa T, Tanaka M, Futai N. On-chip incubation system for long-term microfluidic cell culture. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. p. 8404-8407. 6092073 https://doi.org/10.1109/IEMBS.2011.6092073
Takano, Atsushi ; Ogawa, Tomohisa ; Tanaka, Masato ; Futai, Nobuyuki. / On-chip incubation system for long-term microfluidic cell culture. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. pp. 8404-8407
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