On-chip incubation system for long-term microfluidic cell culture.

Atsushi Takano, Tomohisa Ogawa, Masato Tanaka, Nobuyuki Futai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within < 3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days.

Original languageEnglish
Pages (from-to)8404-8407
Number of pages4
JournalConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
Volume2011
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Cell culture
Microfluidics
Polydimethylsiloxane
Sodium bicarbonate
Gas supply
Water
Carbonates
Microscopes
Sodium
Glass
System-on-chip

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

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AU - Ogawa, Tomohisa

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AU - Futai, Nobuyuki

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