On-chip multi-gas incubation for microfluidic cell cultures under hypoxia

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We developed a simple system that regulates CO2 and O2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO2 and desired pO2 levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth.

Original languageEnglish
Article number061101
JournalBiomicrofluidics
Volume8
Issue number6
DOIs
Publication statusPublished - 2014 Nov 25

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jackets
hypoxia
Cell culture
Microfluidics
Gases
chips
Water
embryos
Cell death
cells
gases
death
water
Oxygen
formulations
oxygen

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

On-chip multi-gas incubation for microfluidic cell cultures under hypoxia. / Takano, Atsushi; Tanaka, Masato; Futai, Nobuyuki.

In: Biomicrofluidics, Vol. 8, No. 6, 061101, 25.11.2014.

Research output: Contribution to journalArticle

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