On-chip temperature control under suspended microbridge for cell immobilization and culture system

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1683-1685
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju
Duration: 2009 Nov 12009 Nov 5

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CityJeju
Period09/11/109/11/5

Fingerprint

Cell immobilization
Cell culture
Temperature control
Gels
Yeast
Micromachining
Gelation
Microchannels
Tin oxides
Microfluidics
Hydrogels
Indium
Hydrogel
Dyes
Fluorescent Dyes
Cells
Fabrication
poly-N-isopropylacrylamide
Temperature

Keywords

  • Cell Immobilization
  • ITO
  • PDMS bridge
  • PNIPAAm gel

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Yamanishi, Y., Teramoto, J., Magariyama, Y., Ishihama, A., Fukuda, T., & Arai, F. (2009). On-chip temperature control under suspended microbridge for cell immobilization and culture system. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1683-1685). Chemical and Biological Microsystems Society.

On-chip temperature control under suspended microbridge for cell immobilization and culture system. / Yamanishi, Yoko; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1683-1685.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamanishi, Y, Teramoto, J, Magariyama, Y, Ishihama, A, Fukuda, T & Arai, F 2009, On-chip temperature control under suspended microbridge for cell immobilization and culture system. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1683-1685, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, 09/11/1.
Yamanishi Y, Teramoto J, Magariyama Y, Ishihama A, Fukuda T, Arai F. On-chip temperature control under suspended microbridge for cell immobilization and culture system. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1683-1685
Yamanishi, Yoko ; Teramoto, Jun ; Magariyama, Yukio ; Ishihama, Akira ; Fukuda, Toshio ; Arai, Fumihito. / On-chip temperature control under suspended microbridge for cell immobilization and culture system. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1683-1685
@inproceedings{35199533d8cd4a83bd92415824f35e45,
title = "On-chip temperature control under suspended microbridge for cell immobilization and culture system",
abstract = "We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.",
keywords = "Cell Immobilization, ITO, PDMS bridge, PNIPAAm gel",
author = "Yoko Yamanishi and Jun Teramoto and Yukio Magariyama and Akira Ishihama and Toshio Fukuda and Fumihito Arai",
year = "2009",
language = "English",
isbn = "9780979806421",
pages = "1683--1685",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - On-chip temperature control under suspended microbridge for cell immobilization and culture system

AU - Yamanishi, Yoko

AU - Teramoto, Jun

AU - Magariyama, Yukio

AU - Ishihama, Akira

AU - Fukuda, Toshio

AU - Arai, Fumihito

PY - 2009

Y1 - 2009

N2 - We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

AB - We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

KW - Cell Immobilization

KW - ITO

KW - PDMS bridge

KW - PNIPAAm gel

UR - http://www.scopus.com/inward/record.url?scp=84901770876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901770876&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84901770876

SN - 9780979806421

SP - 1683

EP - 1685

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

ER -