On-chip temperature sensing and control for cell immobilization and culture system

Yoko Yamanishi, Edward Chow, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper presents a temperature sensing and controlling microfluid chip for cell immobilization using a thermo-sensitive hydrogel (PNIPAAm). The ITO (Indium Tin Oxide) microheaters fabricated by micromachining technology, perform heating of the solution of cell, PNIPAAm and Calcein-AM (fluorescent dye) in microchannels. It is important to note that a thick gel formation cause to block the observation of culturing cells due to the autofluorescence of gel which reduce the S/N ratio. For the present study, we are targeting to fabricate a suspended micro-bridge above a microheater to limit the height of gel to form "thin and transparent gel layer" above the heater. The research was focused on controlling of gel thickness which eliminated unnecessary interferences of autofluorescence of gel in the observation by the inverted microscope, and a better S/N ratio can be obtained than the conventional observation with uncontrolled gel thickness. All the heating and a suspended biocompatible micro-bridge were integrated on a chip, in which yeast cells immobilization can be performed by the gelation of the PNIPAAm solution.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
Pages295-300
Number of pages6
DOIs
Publication statusPublished - 2008
Externally publishedYes
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya
Duration: 2008 Nov 62008 Nov 9

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CityNagoya
Period08/11/608/11/9

Fingerprint

Cell immobilization
Cell culture
Gels
Temperature
Heating
Micromachining
Gelation
Microchannels
Tin oxides
Hydrogels
Yeast
Indium
Microscopes
Dyes

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

Cite this

Yamanishi, Y., Chow, E., Teramoto, J., Magariyama, Y., Ishihama, A., Fukuda, T., & Arai, F. (2008). On-chip temperature sensing and control for cell immobilization and culture system. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008 (pp. 295-300). [4752466] https://doi.org/10.1109/MHS.2008.4752466

On-chip temperature sensing and control for cell immobilization and culture system. / Yamanishi, Yoko; Chow, Edward; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008. 2008. p. 295-300 4752466.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamanishi, Y, Chow, E, Teramoto, J, Magariyama, Y, Ishihama, A, Fukuda, T & Arai, F 2008, On-chip temperature sensing and control for cell immobilization and culture system. in 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008., 4752466, pp. 295-300, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation", Nagoya, 08/11/6. https://doi.org/10.1109/MHS.2008.4752466
Yamanishi Y, Chow E, Teramoto J, Magariyama Y, Ishihama A, Fukuda T et al. On-chip temperature sensing and control for cell immobilization and culture system. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008. 2008. p. 295-300. 4752466 https://doi.org/10.1109/MHS.2008.4752466
Yamanishi, Yoko ; Chow, Edward ; Teramoto, Jun ; Magariyama, Yukio ; Ishihama, Akira ; Fukuda, Toshio ; Arai, Fumihito. / On-chip temperature sensing and control for cell immobilization and culture system. 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008. 2008. pp. 295-300
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