Optical counting of trapped bacteria in dielectrophoretic microdevice with pillar array

Satoshi Uchida, Ryota Nakao, Chihiro Asai, Takayuki Jin, Yasuharu Shiine, Hiroyuki Nishikawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The development of rapid, accurate and simple screening system is essential in actual processes for large-volume water treatment from a standpoint of recent strict regulations on environmental water pollution. in the present work, we newly produced a dielectrophoretic microdevice with three dimensional microstructures and sophisticated microchannel to trap and detect bacteria efficiently. We measured the fluorescence area and intensity of stained bacteria in the gap between electrodes, and estimated the number of trapped bacteria quantitatively. The dependence of the trapping efficiency of bacteria on flow velocity was also discussed.

Original languageEnglish
Pages (from-to)165-176
Number of pages12
JournalIntelligent Automation and Soft Computing
Volume18
Issue number2
DOIs
Publication statusPublished - 2012 Jan

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Keywords

  • Bacteria
  • Dielectrophoresis
  • Fluorescence spectroscopy
  • Microdevice
  • Pillar array
  • Proton beam writing

ASJC Scopus subject areas

  • Software
  • Theoretical Computer Science
  • Computational Theory and Mathematics
  • Artificial Intelligence

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