Optical counting of trapped bacteria in dielectrophoretic microdevice with pillar array

Satoshi Uchida, Ryota Nakao, Chihiro Asai, Takayuki Jin, Yasuharu Shiine, Hiroyuki Nishikawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The development of rapid, accurate and simple screening system is essential in actual processes for large-volume water treatment from a standpoint of recent strict regulations on environmental water pollution. In the present work, we newly produced a dielectrophoretic microdevice with three dimensional microstructures and sophisticated microchannel to trap and detect bacteria efficiently. We measured the fluorescence area and intensity of stained bacteria in the gap between electrodes, and estimated the number of trapped bacteria quantitatively. The dependence of the trapping efficiency of bacteria on flow velocity was also discussed.

Original languageEnglish
Pages (from-to)165-176
Number of pages12
JournalIntelligent Automation and Soft Computing
Volume18
Issue number2
Publication statusPublished - 2012

Fingerprint

Bacteria
Water pollution
Microchannels
Water treatment
Flow velocity
Screening
Fluorescence
Microstructure
Electrodes

Keywords

  • Bacteria
  • Dielectrophoresis
  • Fluorescence spectroscopy
  • Microdevice
  • Pillar array
  • Proton beam writing

ASJC Scopus subject areas

  • Artificial Intelligence
  • Software
  • Theoretical Computer Science
  • Computational Theory and Mathematics

Cite this

Optical counting of trapped bacteria in dielectrophoretic microdevice with pillar array. / Uchida, Satoshi; Nakao, Ryota; Asai, Chihiro; Jin, Takayuki; Shiine, Yasuharu; Nishikawa, Hiroyuki.

In: Intelligent Automation and Soft Computing, Vol. 18, No. 2, 2012, p. 165-176.

Research output: Contribution to journalArticle

Uchida, Satoshi ; Nakao, Ryota ; Asai, Chihiro ; Jin, Takayuki ; Shiine, Yasuharu ; Nishikawa, Hiroyuki. / Optical counting of trapped bacteria in dielectrophoretic microdevice with pillar array. In: Intelligent Automation and Soft Computing. 2012 ; Vol. 18, No. 2. pp. 165-176.
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