TY - JOUR
T1 - Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH)
AU - Chien, Po Jen
AU - Ye, Ming
AU - Suzuki, Takuma
AU - Toma, Koji
AU - Arakawa, Takahiro
AU - Iwasaki, Yasuhiko
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work was partly supported by Tokyo Medical and Dental University Scholarship (Sony Corporation supported), Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 26280053 , by Japan Science and Technology Agency (JST) and by Ministry of Education, Culture, Sports, Science and Technology (MEXT) .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Isopropanol (IPA) is an important solvent used in industrial activity often found in hospitals as antiseptic alcohol rub. Also, IPA may have the potential to be a biomarker of diabetic ketoacidosis. In this study, an optical biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) for IPA measurement was constructed and evaluated. An ultraviolet light emitting diode (UV-LED, λ=340 nm) was employed as the excitation light to excite nicotinamide adenine dinucleotide (NADH). A photomultiplier tube (PMT) was connected to a two-way branch optical fiber for measuring the fluorescence emitted from the NADH. S-ADH was immobilized on the membrane to catalyze IPA to acetone and reduce NAD+ to be NADH. This IPA biosensor shows highly sensitivity and selectivity, the calibration range is from 500 nmol L−1 to 1 mmol L−1. The optimization of buffer pH, temperature, and the enzyme-immobilized method were also evaluated. The detection of IPA in nail related cosmetic using our IPA biosensor was also carried out. The results showed that large amounts of IPA were used in these kinds of cosmetics. This IPA biosensor comes with the advantages of rapid reaction, good reproducibility, and wide dynamic range, and is also expected to use for clinical IPA detections in serum or other medical and health related applications.
AB - Isopropanol (IPA) is an important solvent used in industrial activity often found in hospitals as antiseptic alcohol rub. Also, IPA may have the potential to be a biomarker of diabetic ketoacidosis. In this study, an optical biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) for IPA measurement was constructed and evaluated. An ultraviolet light emitting diode (UV-LED, λ=340 nm) was employed as the excitation light to excite nicotinamide adenine dinucleotide (NADH). A photomultiplier tube (PMT) was connected to a two-way branch optical fiber for measuring the fluorescence emitted from the NADH. S-ADH was immobilized on the membrane to catalyze IPA to acetone and reduce NAD+ to be NADH. This IPA biosensor shows highly sensitivity and selectivity, the calibration range is from 500 nmol L−1 to 1 mmol L−1. The optimization of buffer pH, temperature, and the enzyme-immobilized method were also evaluated. The detection of IPA in nail related cosmetic using our IPA biosensor was also carried out. The results showed that large amounts of IPA were used in these kinds of cosmetics. This IPA biosensor comes with the advantages of rapid reaction, good reproducibility, and wide dynamic range, and is also expected to use for clinical IPA detections in serum or other medical and health related applications.
KW - Biosensor
KW - Fiber-optic
KW - Isopropanol
KW - NADH
KW - Secondary alcohol dehydrogenase
KW - UV-LED
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U2 - 10.1016/j.talanta.2016.06.036
DO - 10.1016/j.talanta.2016.06.036
M3 - Article
C2 - 27474326
AN - SCOPUS:84979037358
SN - 0039-9140
VL - 159
SP - 418
EP - 424
JO - Talanta
JF - Talanta
ER -