Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology

Kabiru Ibrahim Karamba, Siti Aqlima Ahmad, Azham Bin Zulkharnain, Mohd Arif Syed, Khalilah Abdul Khalil, Nor Aripin Shamaan, Farrah Aini Dahalan, Mohd Yunus Shukor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Gold mining companies are known to use cyanide to extract gold from minerals. The indiscriminate use of cyanide presents a major environmental issue. Serratia marcescens strain AQ07 was found to have cyanide-degrading ability. Optimisation of biodegradation condition was carried out utilising one factor at a time and response surface methodology. Cyanide degradation corresponded with growth rate with a maximum growth rate of 16.14 log cfu/mL on day 3 of incubation. Glucose and yeast extract are suitable carbon and nitrogen sources. Six parameters including carbon and nitrogen sources, pH, temperature, inoculum size and cyanide concentration were optimised. In line with the central composite design of response surface methodology, cyanide degradation was optimum at glucose concentration 5.5 g/L, yeast extract 0.55 g/L, pH 6, temperature 32.5 °C, inoculum size 20 % and cyanide concentration 200 mg/L. It was able to stand cyanide toxicity of up to 700 mg/L, which makes it an important candidate for bioremediation of cyanide. The bacterium was observed to degrade 95.6 % of 200 mg/L KCN under the optimised condition. Bacteria are reported to degrade cyanide into ammonia, formamide or formate and carbon dioxide, which are less toxic by-products. These bacteria illustrate good cyanide degradation potential that can be harnessed in cyanide remediation.

Original languageEnglish
Pages (from-to)533-545
Number of pages13
JournalRendiconti Lincei
Volume27
Issue number3
DOIs
Publication statusPublished - 2016 Sep 1
Externally publishedYes

Fingerprint

Serratia marcescens
cyanides
cyanide
response surface methodology
biodegradation
methodology
yeast extract
gold
degradation
bacterium
yeast
removal
bacteria
inoculum
glucose
formates
carbon
nitrogen
bioremediation
remediation

Keywords

  • Biodegradation
  • Cyanide
  • One factor at a time (OFAT)
  • Response surface methodology (RSM)
  • Serratia marcescens

ASJC Scopus subject areas

  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)
  • Earth and Planetary Sciences(all)

Cite this

Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology. / Karamba, Kabiru Ibrahim; Ahmad, Siti Aqlima; Bin Zulkharnain, Azham; Syed, Mohd Arif; Khalil, Khalilah Abdul; Shamaan, Nor Aripin; Dahalan, Farrah Aini; Shukor, Mohd Yunus.

In: Rendiconti Lincei, Vol. 27, No. 3, 01.09.2016, p. 533-545.

Research output: Contribution to journalArticle

Karamba, Kabiru Ibrahim ; Ahmad, Siti Aqlima ; Bin Zulkharnain, Azham ; Syed, Mohd Arif ; Khalil, Khalilah Abdul ; Shamaan, Nor Aripin ; Dahalan, Farrah Aini ; Shukor, Mohd Yunus. / Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology. In: Rendiconti Lincei. 2016 ; Vol. 27, No. 3. pp. 533-545.
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AU - Syed, Mohd Arif

AU - Khalil, Khalilah Abdul

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