Abstract
An electrochemical biosensor based on a carbon nanotube (CNT) layer with glucose oxidase (GQx) enzyme sandwiched between 6-nm-thick acetonitrile plasma-polymerized films (PPFs) was fabricated. The lower PPF was deposited onto a sputtered gold electrode. The optimization of the casting formation of the CNT layer onto the lower PPF is addressed. As a result, the best candidate for the casting solution was a 1:1 mixture of phosphate buffer and ethanol, and the optimized CNT concentration was 7.5 mg ml-1. The optimized glucose biosensor characteristics exhibited ultrasensitivity (a sensitivity of 11 μA mM-1 cm-2 (4.9-19 mM), a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (< 4 s to reach 95% of maximum response). This high performance is attributed to the excellent electrocatalytic activity and enhancement of electron transfer that CNTs provide, and because the PPF and/or plasma process for CNTs are an enzyme-friendly platform.
| Original language | English |
|---|---|
| Pages (from-to) | 289-298 |
| Number of pages | 10 |
| Journal | Sensors and Materials |
| Volume | 20 |
| Issue number | 6 |
| Publication status | Published - 2008 |
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Keywords
- Amperometric biosensor
- Carbon nanotube
- Glucose oxidase
- Plasma-polymerized film
ASJC Scopus subject areas
- Instrumentation
- Materials Science(all)
Cite this
Optimization of carbon nanotube layer formation on plasma-polymerized thin film for enzyme biosensor. / Matsui, Yasunori; Yoshizawa, Masahiro; Hoshino, Tatsuya; Muguruma, Hitoshi.
In: Sensors and Materials, Vol. 20, No. 6, 2008, p. 289-298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of carbon nanotube layer formation on plasma-polymerized thin film for enzyme biosensor
AU - Matsui, Yasunori
AU - Yoshizawa, Masahiro
AU - Hoshino, Tatsuya
AU - Muguruma, Hitoshi
PY - 2008
Y1 - 2008
N2 - An electrochemical biosensor based on a carbon nanotube (CNT) layer with glucose oxidase (GQx) enzyme sandwiched between 6-nm-thick acetonitrile plasma-polymerized films (PPFs) was fabricated. The lower PPF was deposited onto a sputtered gold electrode. The optimization of the casting formation of the CNT layer onto the lower PPF is addressed. As a result, the best candidate for the casting solution was a 1:1 mixture of phosphate buffer and ethanol, and the optimized CNT concentration was 7.5 mg ml-1. The optimized glucose biosensor characteristics exhibited ultrasensitivity (a sensitivity of 11 μA mM-1 cm-2 (4.9-19 mM), a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (< 4 s to reach 95% of maximum response). This high performance is attributed to the excellent electrocatalytic activity and enhancement of electron transfer that CNTs provide, and because the PPF and/or plasma process for CNTs are an enzyme-friendly platform.
AB - An electrochemical biosensor based on a carbon nanotube (CNT) layer with glucose oxidase (GQx) enzyme sandwiched between 6-nm-thick acetonitrile plasma-polymerized films (PPFs) was fabricated. The lower PPF was deposited onto a sputtered gold electrode. The optimization of the casting formation of the CNT layer onto the lower PPF is addressed. As a result, the best candidate for the casting solution was a 1:1 mixture of phosphate buffer and ethanol, and the optimized CNT concentration was 7.5 mg ml-1. The optimized glucose biosensor characteristics exhibited ultrasensitivity (a sensitivity of 11 μA mM-1 cm-2 (4.9-19 mM), a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (< 4 s to reach 95% of maximum response). This high performance is attributed to the excellent electrocatalytic activity and enhancement of electron transfer that CNTs provide, and because the PPF and/or plasma process for CNTs are an enzyme-friendly platform.
KW - Amperometric biosensor
KW - Carbon nanotube
KW - Glucose oxidase
KW - Plasma-polymerized film
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M3 - Article
AN - SCOPUS:58149242830
VL - 20
SP - 289
EP - 298
JO - Sensors and Materials
JF - Sensors and Materials
SN - 0914-4935
IS - 6
ER -