Optimization of islet microencapsulation with thin polymer membranes for long-term stability

Shota Toda, Artin Fattah, Kenta Asawa, Naoko Nakamura, Kristina N. Ekdahl, Bo Nilsson, Yuji Teramura

Research output: Contribution to journalArticle

Abstract

Microencapsulation of islets can protect against immune reactions from the host immune system after transplantation. However, sufficient numbers of islets cannot be transplanted due to the increase of the size and total volume. Therefore, thin and stable polymer membranes are required for the microencapsulation. Here, we undertook the cell microencapsulation using poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) and layer-by-layer membrane of multiple-arm PEG. In order to examine the membrane stability, we used different molecular weights of 4-arm PEG (10k, 20k and 40k)-Mal to examine the influence on the polymer membrane stability. We found that the polymer membrane made of 4-arm PEG(40k)-Mal showed the highest stability on the cell surface. Also, the polymer membrane did not disturb the insulin secretion from beta cells.

Original languageEnglish
Article number755
JournalMicromachines
Volume10
Issue number11
DOIs
Publication statusPublished - 2019 Nov 1

Keywords

  • Bioartificial pancreas
  • Cell surface modification
  • Islet transplantation
  • Microencapsulation
  • Polyethylene glycol-lipid (PEG-lipid)

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Toda, S., Fattah, A., Asawa, K., Nakamura, N., Ekdahl, K. N., Nilsson, B., & Teramura, Y. (2019). Optimization of islet microencapsulation with thin polymer membranes for long-term stability. Micromachines, 10(11), [755]. https://doi.org/10.3390/mi10110755