Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response

Yoshitaka Nakayama, Kenjiro Yoshimura, Hidetoshi Iida

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A key molecule of sensing machineries essential for survival upon hypo-osmotic shock is the mechanosensitive channel. The bacterial mechanosensitive channel MscS functions directly for this purpose by releasing cytoplasmic solutes out of the cell, whereas plant MscS homologues are found to function in chloroplast organization. Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism different from that operated by the bacterial MscS. Upon hypo-osmotic shock, msy2 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells display greater cell swelling than wild-type cells and undergo cell death. Cell swelling precedes an intracellular Ca 2+ increase, which was greater in msy1 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells than in wild-type cells. Fluorescent microscopy showed that Msy1 and Msy2 localize mainly to the endoplasmic reticulum. These observations suggest that organellar Msy1 and Msy2 regulate intracellular Ca 2+ and cell volume for survival upon hypo-osmotic shock.

Original languageEnglish
Article number1020
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

yeast
Yeast
fission
shock
cells
Swelling
Cell death
swelling
Microscopic examination
chloroplasts
endoplasmic reticulum
Molecules
Osmotic Pressure
releasing
death
solutes
microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response. / Nakayama, Yoshitaka; Yoshimura, Kenjiro; Iida, Hidetoshi.

In: Nature Communications, Vol. 3, 1020, 2012.

Research output: Contribution to journalArticle

@article{286df015c7e54313ae5e413fa1eff531,
title = "Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response",
abstract = "A key molecule of sensing machineries essential for survival upon hypo-osmotic shock is the mechanosensitive channel. The bacterial mechanosensitive channel MscS functions directly for this purpose by releasing cytoplasmic solutes out of the cell, whereas plant MscS homologues are found to function in chloroplast organization. Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism different from that operated by the bacterial MscS. Upon hypo-osmotic shock, msy2 ĝ€{"} and msy1 ĝ€{"} msy2 ĝ€{"} cells display greater cell swelling than wild-type cells and undergo cell death. Cell swelling precedes an intracellular Ca 2+ increase, which was greater in msy1 ĝ€{"} and msy1 ĝ€{"} msy2 ĝ€{"} cells than in wild-type cells. Fluorescent microscopy showed that Msy1 and Msy2 localize mainly to the endoplasmic reticulum. These observations suggest that organellar Msy1 and Msy2 regulate intracellular Ca 2+ and cell volume for survival upon hypo-osmotic shock.",
author = "Yoshitaka Nakayama and Kenjiro Yoshimura and Hidetoshi Iida",
year = "2012",
doi = "10.1038/ncomms2014",
language = "English",
volume = "3",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response

AU - Nakayama, Yoshitaka

AU - Yoshimura, Kenjiro

AU - Iida, Hidetoshi

PY - 2012

Y1 - 2012

N2 - A key molecule of sensing machineries essential for survival upon hypo-osmotic shock is the mechanosensitive channel. The bacterial mechanosensitive channel MscS functions directly for this purpose by releasing cytoplasmic solutes out of the cell, whereas plant MscS homologues are found to function in chloroplast organization. Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism different from that operated by the bacterial MscS. Upon hypo-osmotic shock, msy2 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells display greater cell swelling than wild-type cells and undergo cell death. Cell swelling precedes an intracellular Ca 2+ increase, which was greater in msy1 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells than in wild-type cells. Fluorescent microscopy showed that Msy1 and Msy2 localize mainly to the endoplasmic reticulum. These observations suggest that organellar Msy1 and Msy2 regulate intracellular Ca 2+ and cell volume for survival upon hypo-osmotic shock.

AB - A key molecule of sensing machineries essential for survival upon hypo-osmotic shock is the mechanosensitive channel. The bacterial mechanosensitive channel MscS functions directly for this purpose by releasing cytoplasmic solutes out of the cell, whereas plant MscS homologues are found to function in chloroplast organization. Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism different from that operated by the bacterial MscS. Upon hypo-osmotic shock, msy2 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells display greater cell swelling than wild-type cells and undergo cell death. Cell swelling precedes an intracellular Ca 2+ increase, which was greater in msy1 ĝ€" and msy1 ĝ€" msy2 ĝ€" cells than in wild-type cells. Fluorescent microscopy showed that Msy1 and Msy2 localize mainly to the endoplasmic reticulum. These observations suggest that organellar Msy1 and Msy2 regulate intracellular Ca 2+ and cell volume for survival upon hypo-osmotic shock.

UR - http://www.scopus.com/inward/record.url?scp=84866138261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866138261&partnerID=8YFLogxK

U2 - 10.1038/ncomms2014

DO - 10.1038/ncomms2014

M3 - Article

C2 - 22910366

AN - SCOPUS:84866138261

VL - 3

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 1020

ER -