Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita, Hiroaki Suzuki, Hisao Masai, Kiyohisa Mizumoto, Etsuro Ogata, Ikuo Nishimoto, Sadakazu Aiso, Masaaki Matsuoka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

Original languageEnglish
Pages (from-to)554-561
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume324
Issue number2
DOIs
Publication statusPublished - 2004 Nov 12

Keywords

  • CR
  • Cdc7
  • Cell cycle
  • Periphilin
  • S-phase arrest

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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