Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita, Hiroaki Suzuki, Hisao Masai, Kiyohisa Mizumoto, Etsuro Ogata, Ikuo Nishimoto, Sadakazu Aiso, Masaaki Matsuoka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

Original languageEnglish
Pages (from-to)554-561
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume324
Issue number2
DOIs
Publication statusPublished - 2004 Nov 12
Externally publishedYes

Fingerprint

DNA
Nuclear Proteins
Genes
Messenger RNA
Proteins

Keywords

  • Cdc7
  • Cell cycle
  • CR
  • Periphilin
  • S-phase arrest

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kurita, M., Suzuki, H., Masai, H., Mizumoto, K., Ogata, E., Nishimoto, I., ... Matsuoka, M. (2004). Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. Biochemical and Biophysical Research Communications, 324(2), 554-561. https://doi.org/10.1016/j.bbrc.2004.09.083

Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. / Kurita, Megumi; Suzuki, Hiroaki; Masai, Hisao; Mizumoto, Kiyohisa; Ogata, Etsuro; Nishimoto, Ikuo; Aiso, Sadakazu; Matsuoka, Masaaki.

In: Biochemical and Biophysical Research Communications, Vol. 324, No. 2, 12.11.2004, p. 554-561.

Research output: Contribution to journalArticle

Kurita, Megumi ; Suzuki, Hiroaki ; Masai, Hisao ; Mizumoto, Kiyohisa ; Ogata, Etsuro ; Nishimoto, Ikuo ; Aiso, Sadakazu ; Matsuoka, Masaaki. / Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 324, No. 2. pp. 554-561.
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