Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase

Hiroyuki Fuse, Misaki Ohta, Osamu Takimura, Katsuji Murakami, Hiroyuki Inoue, Yukiho Yamaoka, Jose M. Oclarit, Toshio Omori

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmo X gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.

Original languageEnglish
Pages (from-to)1925-1931
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number10
Publication statusPublished - 1998 Oct
Externally publishedYes

Fingerprint

methane monooxygenase
Methylomicrobium
dimethyl sulfide
trichloroethylene
Trichloroethylene
Methane
oxidation
Oxidation
Genes
methanotrophs
Dimethyl sulfoxide
dimethyl sulfoxide
Methylomicrobium pelagicum
Dimethyl Sulfoxide
genes
naphthalene
mixed culture
Naphthalene
Ribosomal DNA
Amino acids

Keywords

  • Dimethyl sulfide
  • Marine methanotroph
  • Methylomicrobium
  • Soluble methane monooxygenase
  • Trichloroethylene

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase. / Fuse, Hiroyuki; Ohta, Misaki; Takimura, Osamu; Murakami, Katsuji; Inoue, Hiroyuki; Yamaoka, Yukiho; Oclarit, Jose M.; Omori, Toshio.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 10, 10.1998, p. 1925-1931.

Research output: Contribution to journalArticle

Fuse, H, Ohta, M, Takimura, O, Murakami, K, Inoue, H, Yamaoka, Y, Oclarit, JM & Omori, T 1998, 'Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase', Bioscience, Biotechnology and Biochemistry, vol. 62, no. 10, pp. 1925-1931.
Fuse, Hiroyuki ; Ohta, Misaki ; Takimura, Osamu ; Murakami, Katsuji ; Inoue, Hiroyuki ; Yamaoka, Yukiho ; Oclarit, Jose M. ; Omori, Toshio. / Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase. In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 10. pp. 1925-1931.
@article{043133641ada4995b72fadaf38899b93,
title = "Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase",
abstract = "Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmo X gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.",
keywords = "Dimethyl sulfide, Marine methanotroph, Methylomicrobium, Soluble methane monooxygenase, Trichloroethylene",
author = "Hiroyuki Fuse and Misaki Ohta and Osamu Takimura and Katsuji Murakami and Hiroyuki Inoue and Yukiho Yamaoka and Oclarit, {Jose M.} and Toshio Omori",
year = "1998",
month = "10",
language = "English",
volume = "62",
pages = "1925--1931",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "10",

}

TY - JOUR

T1 - Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase

AU - Fuse, Hiroyuki

AU - Ohta, Misaki

AU - Takimura, Osamu

AU - Murakami, Katsuji

AU - Inoue, Hiroyuki

AU - Yamaoka, Yukiho

AU - Oclarit, Jose M.

AU - Omori, Toshio

PY - 1998/10

Y1 - 1998/10

N2 - Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmo X gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.

AB - Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmo X gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.

KW - Dimethyl sulfide

KW - Marine methanotroph

KW - Methylomicrobium

KW - Soluble methane monooxygenase

KW - Trichloroethylene

UR - http://www.scopus.com/inward/record.url?scp=0032174859&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032174859&partnerID=8YFLogxK

M3 - Article

VL - 62

SP - 1925

EP - 1931

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 10

ER -