Oxidation of trichloroethylene and dimethyl sulfide by a marine methylomicrobium strain containing soluble methane monooxygenase

Hiroyuki Fuse, Misaki Ohta, Osamu Takimura, Katsuji Murakami, Hiroyuki Inoue, Yukiho Yamaoka, Jose M. Oclarit, Toshio Omori

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Abstract

Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmoX gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.

Original languageEnglish
Pages (from-to)1925-1931
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number10
DOIs
Publication statusPublished - 1998 Jan 1

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Keywords

  • Dimethyl sulfide
  • Marine methanotroph
  • Methylomicrobium
  • Soluble methane monooxygenase
  • Trichloroethylene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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