Partial extraction of inner dynein arms from an outer arm-less mutant of Chlamydomonas reinhardtii

Kenjiro Yoshimura, K. Takahashi

Research output: Contribution to journalArticle

Abstract

Flagellar axonemes that lack the outer dynein arms and a part of the inner dynein arms were prepared by extracting demembranated axonemes of an outer-arm less mutant of Chlamydomonas with a solution containing 0.3 M KCl and 10 μM ATP. When reactivated with 1 mM ATP, the extracted axonemes, whose beat frequency before the extraction had been 26.9 ± 2.6 Hz (26°C, n=10), beat with a reduced frequency of 14.9 ± 3.7 Hz (26°C, n=10). The decrease in beat frequency depended on the KCl concentration and on the presence of ATP in the extracting solution. The bend angle and the curvature of bend were not affected by the extraction. Electron microscopic observation showed that 28.5% of the inner arms were weak in appearance or lost in the extracted axoneme. Electrophoresis of the axonemal proteins indicated that one of the high molecular weight polypeptides of the inner dynein arm was reduced in quantity. These results suggest that some part of the inner arms has the function of increasing the flagellar beat frequency but is not essential for flagellar motility.

Original languageEnglish
Pages (from-to)741-749
Number of pages9
JournalCell Structure and Function
Volume14
Issue number6
Publication statusPublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Partial extraction of inner dynein arms from an outer arm-less mutant of Chlamydomonas reinhardtii. / Yoshimura, Kenjiro; Takahashi, K.

In: Cell Structure and Function, Vol. 14, No. 6, 1989, p. 741-749.

Research output: Contribution to journalArticle

@article{4b78aadca05d402188c4cc2484de2934,
title = "Partial extraction of inner dynein arms from an outer arm-less mutant of Chlamydomonas reinhardtii",
abstract = "Flagellar axonemes that lack the outer dynein arms and a part of the inner dynein arms were prepared by extracting demembranated axonemes of an outer-arm less mutant of Chlamydomonas with a solution containing 0.3 M KCl and 10 μM ATP. When reactivated with 1 mM ATP, the extracted axonemes, whose beat frequency before the extraction had been 26.9 ± 2.6 Hz (26°C, n=10), beat with a reduced frequency of 14.9 ± 3.7 Hz (26°C, n=10). The decrease in beat frequency depended on the KCl concentration and on the presence of ATP in the extracting solution. The bend angle and the curvature of bend were not affected by the extraction. Electron microscopic observation showed that 28.5{\%} of the inner arms were weak in appearance or lost in the extracted axoneme. Electrophoresis of the axonemal proteins indicated that one of the high molecular weight polypeptides of the inner dynein arm was reduced in quantity. These results suggest that some part of the inner arms has the function of increasing the flagellar beat frequency but is not essential for flagellar motility.",
author = "Kenjiro Yoshimura and K. Takahashi",
year = "1989",
language = "English",
volume = "14",
pages = "741--749",
journal = "Cell Structure and Function",
issn = "0386-7196",
publisher = "Japan Society for Cell Biology",
number = "6",

}

TY - JOUR

T1 - Partial extraction of inner dynein arms from an outer arm-less mutant of Chlamydomonas reinhardtii

AU - Yoshimura, Kenjiro

AU - Takahashi, K.

PY - 1989

Y1 - 1989

N2 - Flagellar axonemes that lack the outer dynein arms and a part of the inner dynein arms were prepared by extracting demembranated axonemes of an outer-arm less mutant of Chlamydomonas with a solution containing 0.3 M KCl and 10 μM ATP. When reactivated with 1 mM ATP, the extracted axonemes, whose beat frequency before the extraction had been 26.9 ± 2.6 Hz (26°C, n=10), beat with a reduced frequency of 14.9 ± 3.7 Hz (26°C, n=10). The decrease in beat frequency depended on the KCl concentration and on the presence of ATP in the extracting solution. The bend angle and the curvature of bend were not affected by the extraction. Electron microscopic observation showed that 28.5% of the inner arms were weak in appearance or lost in the extracted axoneme. Electrophoresis of the axonemal proteins indicated that one of the high molecular weight polypeptides of the inner dynein arm was reduced in quantity. These results suggest that some part of the inner arms has the function of increasing the flagellar beat frequency but is not essential for flagellar motility.

AB - Flagellar axonemes that lack the outer dynein arms and a part of the inner dynein arms were prepared by extracting demembranated axonemes of an outer-arm less mutant of Chlamydomonas with a solution containing 0.3 M KCl and 10 μM ATP. When reactivated with 1 mM ATP, the extracted axonemes, whose beat frequency before the extraction had been 26.9 ± 2.6 Hz (26°C, n=10), beat with a reduced frequency of 14.9 ± 3.7 Hz (26°C, n=10). The decrease in beat frequency depended on the KCl concentration and on the presence of ATP in the extracting solution. The bend angle and the curvature of bend were not affected by the extraction. Electron microscopic observation showed that 28.5% of the inner arms were weak in appearance or lost in the extracted axoneme. Electrophoresis of the axonemal proteins indicated that one of the high molecular weight polypeptides of the inner dynein arm was reduced in quantity. These results suggest that some part of the inner arms has the function of increasing the flagellar beat frequency but is not essential for flagellar motility.

UR - http://www.scopus.com/inward/record.url?scp=0024847788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024847788&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0024847788

VL - 14

SP - 741

EP - 749

JO - Cell Structure and Function

JF - Cell Structure and Function

SN - 0386-7196

IS - 6

ER -