Photo-control of cell adhesion in a flow-type cell chip

Yuichi Tada, Kimio Sumaru, Shinji Sugiura, Junichi Edahiro, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to conferencePaperpeer-review

Abstract

We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel structure at surface, to a photo-responsive cell culture surface, which was functionalized with poly(N-isopropylacrylamide) containing nitrospiropyran residues, by pressure bonding. After systematic examination of the chip design and cell culture condition, we specified the condition allowing to culture living cells in the micro channel for more than 30 hours. We also confirmed that the irradiation of UV light with the wavelength of 365 nm brought a drastic enhancement of cell adhesion as we confirmed previously in dish-like culture system even in a micro channel of the flow-type chip. Practically we succeeded to introduce living cell only in a required region of a micro channel, and to remove the cells from the other region (Fig.1). Further, by using a fluorescent dye, which stains living cells only, viability of the cells was confirmed to be maintained well even after the procedure of cell adhesion control.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Conference

Conference54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Cell adhesion
  • Cell patterning
  • Micro channel
  • Photo-responsive cell culture surface
  • Photo-responsive polymer

ASJC Scopus subject areas

  • Engineering(all)

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