Abstract
The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.
| Original language | English |
|---|---|
| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Editors | J.D. Enderle |
| Pages | 2819-2820 |
| Number of pages | 2 |
| Volume | 4 |
| Publication status | Published - 2000 |
| Externally published | Yes |
| Event | 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Chicago, IL, United States Duration: 2000 Jul 23 → 2000 Jul 28 |
Other
| Other | 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
|---|---|
| Country | United States |
| City | Chicago, IL |
| Period | 00/7/23 → 00/7/28 |
Fingerprint
Keywords
- Microinjection
- PC12
- Photocatalyst
- Photosensitization
- Titanium oxide
ASJC Scopus subject areas
- Bioengineering
Cite this
Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. / Saito, T. K.; Miyauchi, M.; Muguruma, Hitoshi; Suzuki, T.; Kakuta, N.; Watanabe, T.; Hashimoto, K.; Mabuchi, K.
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. ed. / J.D. Enderle. Vol. 4 2000. p. 2819-2820.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection
AU - Saito, T. K.
AU - Miyauchi, M.
AU - Muguruma, Hitoshi
AU - Suzuki, T.
AU - Kakuta, N.
AU - Watanabe, T.
AU - Hashimoto, K.
AU - Mabuchi, K.
PY - 2000
Y1 - 2000
N2 - The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.
AB - The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.
KW - Microinjection
KW - PC12
KW - Photocatalyst
KW - Photosensitization
KW - Titanium oxide
UR - http://www.scopus.com/inward/record.url?scp=0034441559&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034441559&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0034441559
VL - 4
SP - 2819
EP - 2820
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
A2 - Enderle, J.D.
ER -