Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection

T. K. Saito, M. Miyauchi, Hitoshi Muguruma, T. Suzuki, N. Kakuta, T. Watanabe, K. Hashimoto, K. Mabuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
EditorsJ.D. Enderle
Pages2819-2820
Number of pages2
Volume4
Publication statusPublished - 2000
Externally publishedYes
Event22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Chicago, IL, United States
Duration: 2000 Jul 232000 Jul 28

Other

Other22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CountryUnited States
CityChicago, IL
Period00/7/2300/7/28

Fingerprint

Photocatalysts
Ultraviolet radiation
Membranes
Coatings
Photosensitizing Agents
Titanium oxides
Cell membranes
Manipulators
Rats
Reactive Oxygen Species
Coloring Agents
Dyes
Oxidation
Oxygen
Water
Experiments
titanium dioxide

Keywords

  • Microinjection
  • PC12
  • Photocatalyst
  • Photosensitization
  • Titanium oxide

ASJC Scopus subject areas

  • Bioengineering

Cite this

Saito, T. K., Miyauchi, M., Muguruma, H., Suzuki, T., Kakuta, N., Watanabe, T., ... Mabuchi, K. (2000). Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. In J. D. Enderle (Ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 4, pp. 2819-2820)

Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. / Saito, T. K.; Miyauchi, M.; Muguruma, Hitoshi; Suzuki, T.; Kakuta, N.; Watanabe, T.; Hashimoto, K.; Mabuchi, K.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. ed. / J.D. Enderle. Vol. 4 2000. p. 2819-2820.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saito, TK, Miyauchi, M, Muguruma, H, Suzuki, T, Kakuta, N, Watanabe, T, Hashimoto, K & Mabuchi, K 2000, Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. in JD Enderle (ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 4, pp. 2819-2820, 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Chicago, IL, United States, 00/7/23.
Saito TK, Miyauchi M, Muguruma H, Suzuki T, Kakuta N, Watanabe T et al. Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. In Enderle JD, editor, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 4. 2000. p. 2819-2820
Saito, T. K. ; Miyauchi, M. ; Muguruma, Hitoshi ; Suzuki, T. ; Kakuta, N. ; Watanabe, T. ; Hashimoto, K. ; Mabuchi, K. / Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. editor / J.D. Enderle. Vol. 4 2000. pp. 2819-2820
@inproceedings{13b830a2831445148f6032af75962352,
title = "Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection",
abstract = "The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57{\%} for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43{\%} for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20{\%} for the capillary with the 180nm coating, and 6{\%} for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.",
keywords = "Microinjection, PC12, Photocatalyst, Photosensitization, Titanium oxide",
author = "Saito, {T. K.} and M. Miyauchi and Hitoshi Muguruma and T. Suzuki and N. Kakuta and T. Watanabe and K. Hashimoto and K. Mabuchi",
year = "2000",
language = "English",
volume = "4",
pages = "2819--2820",
editor = "J.D. Enderle",
booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

}

TY - GEN

T1 - Photocatalyst coated capillary increases efficiency of membrane penetration process of microinjection

AU - Saito, T. K.

AU - Miyauchi, M.

AU - Muguruma, Hitoshi

AU - Suzuki, T.

AU - Kakuta, N.

AU - Watanabe, T.

AU - Hashimoto, K.

AU - Mabuchi, K.

PY - 2000

Y1 - 2000

N2 - The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.

AB - The microinjection process has been improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to injection. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Titanium oxide was used as a photocatalytic coating for capillaries in this experiment. It catalyzes reactive oxygen species under UV light and degenerates cell membranes by oxidation. This process was evaluated by injecting water-soluble fluorescent Lucifer Yellow (LY) CH dye into rat pheochromocytoma PC12 cells. The injections were automated by a programmable motorized manipulator. The success rate was 57% for injections done using an 180nm-thick titanium-oxide coated capillary under UV light and 43% for a capillary with a coating 120nm thick. Without UV light, the success rates dropped to 20% for the capillary with the 180nm coating, and 6% for the capillary with the 120nm coating. These results suggest that using the PS-effect with microinjections increases injection-success rates and reduces the rate of cell injury caused by injection.

KW - Microinjection

KW - PC12

KW - Photocatalyst

KW - Photosensitization

KW - Titanium oxide

UR - http://www.scopus.com/inward/record.url?scp=0034441559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034441559&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0034441559

VL - 4

SP - 2819

EP - 2820

BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

A2 - Enderle, J.D.

ER -