Photodynamic assistance increases the efficiency of the process of microinjection in animal cells

Takashi K. Saito, Hitoshi Muguruma, Kunihiko Mabuchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalBiotechnology Letters
Volume24
Issue number4
DOIs
Publication statusPublished - 2002 Mar 9

Keywords

  • Detachment assay
  • Microinjection
  • Photodynamic membrane damage
  • Rat PC12 cell line
  • α-terthienyl derivative

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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